Anti-PDK2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15119

Core Product Specifications and Parameters

Biological Background: PDK2 Function and Localization

• PDK2, also known as pyruvate dehydrogenase kinase isoform 2 or PDHK2, is a mitochondrial kinase encoded by the PDK2 gene in humans. It belongs to the PDK/BCKDK protein kinase family and is expressed in many tissues, with highest levels in heart and skeletal muscle.
• Its core function is to phosphorylate and inactivate the pyruvate dehydrogenase complex (PDC) subunits PDHA1 and PDHA2, thereby regulating the conversion of pyruvate to acetyl-CoA and linking glycolysis to the tricarboxylic acid cycle.
• By inhibiting PDC activity, PDK2 down-regulates aerobic respiration and shifts cellular metabolism towards fatty acid oxidation, playing a key role in metabolic flexibility and adaptation to nutrient availability.
• PDK2 mediates cellular responses to insulin and helps maintain normal blood glucose levels and pH balance, preventing ketone body accumulation during starvation.
• The protein localizes to the mitochondrial matrix, where it directly interacts with the pyruvate dehydrogenase complex.
• Post-translationally, PDK2 is a phosphoprotein; its kinase activity is regulated by various metabolic signals. Alternative splicing produces multiple isoforms.
• Beyond metabolism, PDK2 contributes to cell proliferation control and resistance to apoptosis under oxidative stress, and it modulates p53/TP53-mediated apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within human PDK2 (aa 128-407). For Western blot (WB), consider using lysates from tissues with high PDK2 expression, such as heart or skeletal muscle. A band of approximately 46 kDa is expected.
• For immunofluorescence/immunocytochemistry (IF/ICC), mitochondrial localization should be observed; include appropriate mitochondrial markers as colocalization controls.
• In ELISA applications, the antibody may be used as a capture or detection reagent; optimization of coating concentration and detection conditions is recommended.
• Validate the antibody's reactivity in your specific sample system and application, performing necessary positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDK2 antibody is suitable for research in glucose and fatty acid metabolism, and related metabolic disorders. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to support your assay development and manufacturing needs.