PD-L1/CD274 Rabbit Monoclonal Antibody for FC, ELISA, Inhibition - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• PD-L1 (Programmed cell death 1 ligand 1), also known as B7-H1 or CD274, is a member of the immunoglobulin superfamily that serves as a ligand for the inhibitory receptor PD-1 (PDCD1).
• It plays a central role in immune tolerance by modulating T-cell activation thresholds and limiting effector responses through PD-1 engagement. Related references: PMID:11015443 PMID:28813410 PMID:28813417
• Via an unidentified activating receptor, PD-L1 may costimulate IL-10-producing T-cell subsets. Related references: PMID:10581077
• Under hypoxia, PD-L1 translocates to the nucleus, interacts with phosphorylated STAT3, and promotes transcription of GSDMC, leading to pyroptosis. Related references: PMID:32929201
• Tumor cells exploit the PD-1/PD-L1 axis to suppress anti-tumor immunity; blocking this interaction can restore T-cell function, underpinning cancer immunotherapy. Related references: PMID:28813410 PMID:28813417
• PD-L1 localizes predominantly at the cell membrane but also traffics through early and recycling endosomes; a secreted form and nuclear localization have been observed.
• It is highly expressed in heart, skeletal muscle, placenta, and lung, and on activated immune cells including T cells, B cells, dendritic cells, and monocytes.
• PD-L1 undergoes glycosylation and contains disulfide bonds and immunoglobulin-like domains; it is also subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 1–290), suggesting the antibody recognizes the native receptor-binding region, suitable for flow cytometry and functional blocking studies.
• For ELISA applications, consider pairing with a detection antibody directed against a distinct epitope if developing a sandwich assay.
• Inhibition assays require careful optimization; validate the antibody’s blocking activity in your specific cellular context before use.

CamelBio: Your One-Stop Sourcing Bridge

As your integrated supply partner, CamelBio provides diagnostic manufacturers with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This PD-L1/CD274 monoclonal antibody supports immune-oncology assay development, and our team can source rare targets and tailored raw materials to accelerate your pipeline from concept to clinic.