Anti-OTC Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P00480

Core Product Specifications and Parameters

Biological Background: OTC Function and Localization

• OTC (ornithine transcarbamylase) is a mitochondrial matrix enzyme also known as ornithine carbamoyltransferase or OTCase, encoded by the OTC gene.
• OTC catalyzes the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline, the second step of the urea cycle. Related references: PMID:2556444, PMID:6372096, PMID:8112735
• The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion. Related references: PMID:2556444
• OTC is predominantly expressed in liver and intestinal mucosa.
• The protein is subject to post-translational modifications, including acetylation and phosphorylation.
• UniProt keywords for OTC include transferase, transit peptide, urea cycle, amino-acid biosynthesis, arginine biosynthesis, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 215-354 of human OTC, a region within the mature catalytic domain; this may help minimize cross-reactivity with mitochondrial targeting sequences.
• For Western blotting, the expected molecular weight is approximately 40 kDa; validate band specificity in your sample system.
• For immunoprecipitation (IF-P), ensure appropriate fixation and permeabilization conditions given OTC's mitochondrial matrix localization.
• For ELISA, consider pairing with a capture antibody for sandwich assay development and verify performance in relevant matrices.
• The antibody shows reactivity with human, mouse, and rat; confirm sequence homology and test in your model organism before large-scale use.
• Store the antibody as recommended (shipped on ice bags, typically at -20°C or per manufacturer instructions).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories. For urea cycle and metabolic disease research, the OTC Rabbit mAb is available alongside a broad portfolio of detection reagents and support for rare-target material sourcing. Our network streamlines procurement from concept to clinic, helping you meet assay development timelines efficiently.