Anti-TNF-α Rabbit Monoclonal Antibody for WB, ELISA - P06804

Core Product Specifications and Parameters

Biological Background: TNF-α Function and Localization

• Tumor necrosis factor (TNF-α), also known as cachectin, is a multifunctional cytokine belonging to the tumor necrosis factor ligand superfamily (member 2, TNFSF2). It is primarily secreted by activated macrophages and exerts pleiotropic effects on various cell types.
• TNF-α binds to its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR to mediate cellular responses such as apoptosis, survival, proliferation, and differentiation, depending on the cellular context.
• In adipocytes, TNF-α induces insulin resistance by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake, and by mediating GKAP42 protein degradation. Related references: PMID:25586176
• TNF-α plays a critical role in bone homeostasis by promoting osteoclastogenesis and bone resorption. Related references: PMID:32741026
• It is a potent pyrogen, causing fever either directly or through stimulation of interleukin-1 secretion, and is implicated in the induction of cachexia during chronic inflammation.
• TNF-α exists as both a transmembrane form and a soluble secreted cytokine. The intracellular domain (ICD) form can be generated and has been shown to induce IL-12 production in dendritic cells.
• The protein undergoes several post-translational modifications including glycosylation, myristoylation, phosphorylation, and disulfide bond formation, which regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 80-235) of mouse TNF-α; the antibody may detect both the full-length precursor (~26 kDa) and the cleaved mature soluble form (~17 kDa) in western blotting. Validate in the relevant sample system.
• For sandwich ELISA applications, confirm pairing compatibility with a suitable capture or detection antibody targeting a non-overlapping epitope.
• This antibody exhibits cross-reactivity with rat TNF-α, as indicated. Verify performance in your specific assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for cytokine and inflammation research. Our TNF-α antibody portfolio supports immunoassay development and target validation workflows. Contact us for rare target sourcing and technical consultation.