Anti-JAK1 Rabbit Monoclonal Antibody for WB and ELISA - P23458

Core Product Specifications and Parameters

Biological Background: JAK1 Function and Localization

• JAK1 (JTK3; JAK1A) is a 133 kDa non-receptor tyrosine kinase that belongs to the Janus kinase family and contains SH2 and kinase domains, along with tyrosine-protein kinase and transferase activities.
• It phosphorylates and activates signal-transducing subunits of various cytokine receptors, including IL-2, IL-10, IL-6, IL-31, IFN-alpha/beta/gamma, and oncostatin-M receptor complexes, thereby initiating STAT protein recruitment and signaling. Related references: PMID:11909529, PMID:12133952, PMID:15194700, PMID:16239216, PMID:28111307, PMID:32750333, PMID:7759950, PMID:8232552, PMID:9188471
• Upon interferon binding, JAK1 phosphorylates IFNAR2, creating docking sites for STAT proteins that then become phosphorylated and translocate to the nucleus to activate transcription. Related references: PMID:7759950, PMID:16239216, PMID:32750333, PMID:8232552
• Beyond interferon signaling, JAK1 is necessary for humanin/MT-RNR2-driven STAT3 phosphorylation, CNTF/CLCF1-induced STAT3 activation via LIFR/IL6ST, oncostatin-M signaling through type I and II receptors, and Interleukin-31 signaling through the OSMR/IL31RA complex. Related references: PMID:27384491, PMID:11294841, PMID:9188471, PMID:15194700
• Subcellularly, JAK1 is associated with the endomembrane system, consistent with its role in cytokine receptor signaling at the plasma membrane and intracellular compartments.
• Post-translational modifications include acetylation and ubiquitination (Ubl conjugation); the kinase domain requires ATP and metal ions (Mg²⁺) for activity, and the protein features an SH2 domain and phosphotyrosine residues.
• Tissue specificity data indicate higher expression in primary colon tumors than in normal colon tissue, with metastatic tumors showing expression levels comparable to normal tissue.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to aa 600-700 of human JAK1 (P23458), a region that may be part of the pseudokinase domain or interdomain linker; consider verifying the antibody specificity by peptide competition or knockout validation.
• For Western blot, the predicted molecular weight is ~133 kDa; optimize loading controls and blocking conditions to minimize non-specific binding, especially in immunoprecipitation or complex lysates.
• In ELISA, ensure the coating antigen presents the epitope effectively; test a range of antibody dilutions to determine optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat is anticipated based on supplier data, but validation in species-specific cell or tissue lysates is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research laboratories by providing integrated access to high-quality IVD raw materials, including validated antibody pairs and monoclonal antibodies for cytokine signaling targets like JAK1. Alongside this JAK1 rabbit monoclonal antibody, CamelBio offers bulk ancillary reagents, custom antibody development, and rare target sourcing to support your entire workflow—from early-stage research to clinical assay development.