Anti-MVP Rabbit Monoclonal Antibody for WB & ELISA - Q14764

Core Product Specifications and Parameters

| Product Name | MVP Rabbit mAb | | Remarks/Alias | LRP; VAULT1; MVP | | Species | Human | | GeneID (human) | 9961 | | GeneID | 9961 | | Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MVP (Q14764) | | Source | Rabbit | | Category | Monoclonal Antibodies | | Application | WB, ELISA | | Cross Reactivity | Human, Mouse, Rat | | SWISS | Q14764 | | Protein Weight | 99kDa | | Shipping | Ice bag |

Biological Background: MVP Function and Localization

• MVP is the major structural component of vault ribonucleoprotein complexes, which are involved in cargo transport and signal transduction. Related references: PMID:40691181
• MVP oligomerizes to form a cage-like vault structure that can encapsulate minor vault components such as PARP4. Related references: PMID:40691181
• Vault particles are thought to participate in nucleo-cytoplasmic transport, potentially shuttling cargo between the nucleus and cytoplasm. Related references: PMID:15133037
• MVP down-regulates interferon-gamma (IFNG)-mediated STAT1 signaling and subsequent JAK activation, modulating immune signaling pathways. Related references: PMID:16418217
• It also negatively regulates SRC tyrosine kinase activity and downstream MAP kinase signaling cascades, influencing cell proliferation and survival. Related references: PMID:16441665
• Subcellularly, MVP localizes to the cytoplasm, nucleus, nuclear pore complex, and perinuclear region, consistent with its transport functions.
• MVP is expressed in most normal tissues, with higher levels in secretory epithelial cells and cells exposed to xenobiotics. Overexpression is frequently observed in multidrug-resistant cancer cells, highlighting its role in chemoresistance.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, as annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human MVP, corresponding to the N-terminal region. The antibody is likely to recognize an epitope within this region; consider using peptide competition or truncation mutants to confirm specificity.
• For Western blot, MVP migrates at approximately 99 kDa under reducing conditions. Use whole cell or tissue lysates from human, mouse, or rat sources. A single band near 99 kDa is expected; additional bands may reflect post-translational modifications or isoforms.
• For ELISA, the antibody can be used for direct detection or in a sandwich pair with a validated coating antibody. Optimize coating and detection antibody concentrations for maximum signal-to-noise ratio.
• Validate antibody performance in your specific sample system and application, as conditions may vary. Include appropriate positive (e.g., HeLa or multidrug-resistant cell lines) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For assays targeting major vault protein in the context of drug resistance or cell signaling, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare target raw materials, supporting your entire development journey from concept to clinic.