Anti-Rac1/2/3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P63000 / P15153 / P60763

Core Product Specifications and Parameters

Biological Background: Rac1 Function and Localization

• Rac1 is a plasma membrane-associated small GTPase that acts as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states.
• In its active state, Rac1 binds effector proteins to regulate phagocytosis, epithelial cell polarization, neuron adhesion, migration, differentiation, membrane ruffling, and osteoclast ruffled border formation.
  Related references:
  PMID:1643658 PMID:22843693 PMID:23512198
• Rac1 is a subunit of the phagocyte NADPH oxidase complex, essential for superoxide anion production.
  Related references:
  PMID:38355798
• Rac1 stimulates PKN2 kinase activity.
  Related references:
  PMID:9121475
• In neurons, Rac1 is involved in dendritic spine formation, synaptic plasticity, and regulation of GABA(A) receptor stability through PAK1-mediated F-actin stabilization.
  Related references:
  PMID:12695502
• Required for DSG3-mediated cell junction organization and cortical F-actin anchoring.
  Related references:
  PMID:22796473
• Isoform B exhibits accelerated GDP/GTP exchange and impaired GTP hydrolysis, partially restored by GAPs.
  Related references:
  PMID:14625275
• Subcellular localization includes cell membrane, melanosome, cytoplasm, lamellipodium, dendrites, synapse, nucleus, and ruffle membrane. Post-translational modifications such as prenylation, palmitoylation, methylation, phosphorylation, and ubiquitin conjugation regulate membrane targeting and signaling.

Experimental Guidance and Technical Tips

• The recombinant immunogen covers amino acids 1-189 of human RAC1, a region highly conserved among Rac family members; thus, the antibody recognizes Rac1, Rac2, and Rac3.
• For Western blot, expect a band near 21 kDa. Validate in the relevant sample system and consider appropriate controls for active GTP-bound vs. inactive GDP-bound Rac.
• For IHC-P, optimize antigen retrieval conditions and validate staining in appropriate tissue sections known to express Rac proteins.
• For ELISA, the antibody can be explored as a capture or detection reagent; assay conditions should be optimized individually.
• Rac1 undergoes prenylation; ensure sample preparation maintains membrane localization if studying native interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, one-stop supply chain for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-Rac1/2/3 rabbit monoclonal antibody supports the development of assays targeting Rho GTPase signaling, cytoskeletal dynamics, and related pathways. For rare or custom targets, our technical team is available to assist in sourcing specialized raw materials efficiently.