Anti-TOM70 Rabbit Monoclonal Antibody for WB, ELISA - O94826

Core Product Specifications and Parameters

Biological Background: TOM70 Function and Localization

• Mitochondrial import receptor subunit TOM70 (also known as TOMM70A, TOMM70, Tom70) is a key component of the TOM complex, responsible for recognizing and mediating the translocation of mitochondrial preproteins from the cytosol into the mitochondria in a chaperone-dependent manner. Related references: PMID:12526792 PMID:35025629
• Upon viral infection, TOM70 mediates TBK1 and IRF3 activation via MAVS, thereby promoting host antiviral responses against Sendai virus and possibly other pathogens. Related references: PMID:20628368 PMID:25609812 PMID:32728199
• During viral infection, TOM70 recruits the HSP90AA1:IRF3:BAX complex to the mitochondrion, triggering apoptosis as part of the host defense mechanism. Related references: PMID:25609812
• TOM70 is located on the mitochondrial outer membrane, consistent with its role in preprotein import and signal transduction at the organelle surface.
• The protein undergoes various post-translational modifications including acetylation, phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• Keywords from UniProt highlight its involvement in host-virus interaction, mitochondrial transport, and the presence of TPR repeat motifs important for protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 245-608 of human TOM70, targeting the C‑terminal portion. When performing Western blot, expect a band near the calculated molecular weight of 67 kDa, although post‑translational modifications may cause slight shifts.
• This antibody is recommended for WB and ELISA. For ELISA, a recombinant TOM70 fragment (e.g., the immunogen region) may be used as a positive control or standard.
• Cross‑reactivity extends to mouse and rat species. Validate in the desired species by performing appropriate controls and confirm specificity in the relevant tissue or cell line.
• As the antibody was raised against a recombinant fusion protein, native protein detection may be influenced by protein conformation or complex formation. Sample preparation methods (e.g., denaturing vs. native conditions) should be optimized accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers, laboratories, and researchers with high‑quality reagents. This anti‑TOM70 monoclonal antibody is well‑suited for studies in mitochondrial biology, antiviral immunity, and apoptosis, supporting applications such as WB and ELISA. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare‑target raw materials to streamline your development pipeline.