Anti-Il15ra Rabbit Polyclonal Antibody for WB, ELISA - Q60819

Core Product Specifications and Parameters

Biological Background: Interleukin-15 Receptor Subunit Alpha Function and Localization

• Interleukin-15 receptor subunit alpha (IL-15RA) is a high-affinity receptor for interleukin-15 (IL-15), a cytokine that regulates T and natural killer cell activation and proliferation. Related references: PMID:17947230
• IL-15RA can signal both in cis and in trans, where IL-15RA from one cell presents IL-15 to neighboring cells expressing the IL-2 receptor gamma chain (IL2RG). Related references: PMID:17947230
• The receptor is expressed as a transmembrane protein on the cell surface, but it can also be found in the nucleus membrane and secreted into the extracellular space, highlighting its dynamic subcellular localization.
• IL-15RA is widely expressed across tissues, consistent with its role in immune homeostasis.
• In neutrophils, IL-15RA binds and activates the tyrosine kinase SYK upon IL-15 stimulation, and is required for IL-15-induced phagocytosis in a SYK-dependent manner.
• Structurally, IL-15RA contains a sushi domain (short consensus repeat), and undergoes post-translational modifications including N-linked glycosylation, disulfide bond formation, and phosphorylation.
• Alternative splicing generates multiple isoforms of IL-15RA, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an extracellular domain sequence; the polyclonal antibody may recognize both soluble and membrane-bound forms of IL-15RA.
• For Western blot, consider testing both reducing and non-reducing conditions to evaluate disulfide-dependent epitopes and detect different isoforms.
• In ELISA, the antibody can potentially be used to capture or detect IL-15RA in cell lysates or culture supernatants, but appropriate controls and optimization are advised.
• Validate the antibody in the relevant sample system and application context, as performance may vary depending on target abundance and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with reliable access to raw materials for interleukin-15 receptor-related studies, including this anti-Il15ra polyclonal antibody. As a one-stop IVD raw-material partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your immunoassay development and cytokine research. Let us help you bridge from concept to clinic with quality-assured supplies.