Anti-IL15 Rabbit Polyclonal Antibody for WB and ELISA - P40933

Core Product Specifications and Parameters

Biological Background: IL15 Function and Localization

• Interleukin-15 (IL15) is a cytokine critical for the development of inflammatory and protective immune responses against microbial invaders and parasites, modulating both innate and adaptive immunity (PubMed:15123770). Related references: PMID:15123770
• IL15 stimulates proliferation of natural killer (NK) cells, T-cells, and B-cells, and promotes secretion of various cytokines (PubMed:8178155, PubMed:9326248). Related references: PMID:8178155, PMID:9326248
• In monocytes, IL15 induces production of IL8 and monocyte chemotactic protein 1/CCL2, chemokines that attract neutrophils and monocytes to infection sites (PubMed:9326248). Related references: PMID:9326248
• Unlike most cytokines, IL15 is predominantly expressed on the cell surface in association with its high-affinity receptor IL15RA, signaling to target cells expressing IL2RB and IL2RG (PubMed:10233906, PubMed:23104097, PubMed:8026467). Related references: PMID:10233906, PMID:23104097, PMID:8026467
• IL15 binding triggers JAK1/JAK3 phosphorylation and STAT3/STAT5 activation; in mast cells, it induces STAT6 phosphorylation to control survival and IL4 release (PubMed:7568001). Related references: PMID:7568001
• Subcellular localization includes secretion, cytoplasm, and nucleus, reflecting its multifaceted roles in immune signaling.
• IL15 is most abundant in placenta and skeletal muscle, with lower levels in heart, lung, liver, and kidney; an isoform IL15-S21AA is preferentially expressed in testis and thymus.
• The protein is glycosylated, contains disulfide bonds, and has a molecular weight of approximately 18 kDa (mature form), with precursor forms around 14 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human or mouse immune cells (e.g., peripheral blood mononuclear cells or spleen tissue) where IL15 is endogenously expressed.
• The antibody targets an immunogen spanning amino acids 49-162, which may recognize both precursor (~14 kDa) and mature glycosylated (~18 kDa) forms; validate these bands using appropriate positive controls.
• In ELISA applications, this polyclonal antibody can be used for capture or detection, but pairing with a matched monoclonal antibody or optimizing assay conditions is recommended to ensure specificity.
• Given IL15's association with IL15RA on cell surfaces, consider testing sample preparation methods (e.g., lysis buffers) that preserve surface complexes if studying receptor-bound forms.
• Cross-reactivity with mouse IL15 has been observed, making this antibody suitable for studies in mouse models of inflammation and immunity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to support every stage from concept to clinic. This anti-IL15 polyclonal antibody is well-suited for immune function studies and cytokine detection in diagnostic and research workflows. For bulk orders, custom antibody development, or rare target sourcing, our team delivers flexible and reliable supply solutions.