Anti-IL1RN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P25085

Core Product Specifications and Parameters

Biological Background: IL1RN Function and Localization

• Interleukin-1 receptor antagonist protein (IL1RN, also known as IL-1ra) is a secreted anti-inflammatory cytokine that belongs to the interleukin-1 (IL-1) family.
• IL1RN functions as a competitive antagonist of the proinflammatory cytokines interleukin-1alpha (IL1A) and interleukin-1beta (IL1B), thereby preventing IL1-mediated signal transduction.
• By inhibiting IL1 signaling, IL1RN plays a critical role in protecting against immune dysregulation and uncontrolled systemic inflammation triggered by a broad range of innate stimulatory agents, including pathogens.
• IL1RN is primarily secreted, but a fraction can also be localized in the cytoplasm.
• The protein undergoes post-translational modifications such as glycosylation and disulfide bond formation, and is characterized by an N-terminal signal peptide.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• IL1RN is widely expressed and is essential for maintaining immune homeostasis; its dysregulation is implicated in various autoinflammatory disorders.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 27–178 of mouse IL1RN, encompassing the mature, secreted antagonist domain, and may detect both secreted and cytoplasmic forms.
• For Western blot analysis, a band at approximately 20 kDa can be expected; however, due to glycosylation or other modifications, the observed molecular weight may vary—validate in the relevant sample system.
• This antibody has been documented for use in WB, IF/ICC, and ELISA, and exhibits cross-reactivity with human and mouse IL1RN. Consider optimising dilution and incubation conditions for each application.
• When performing immunofluorescence or immunocytochemistry, appropriate permeabilization protocols should be employed to detect intracellular populations, if present.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B sourcing partner for IVD manufacturers and research laboratories, offering one-stop access to a wide range of raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For studies focused on inflammation and immune regulation, such as those involving IL1RN, we can facilitate procurement of the necessary immunological reagents. Additionally, our team supports rare-target raw-material sourcing to help streamline your development workflow from concept to clinic.