Anti-IL4R Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24394

Core Product Specifications and Parameters

Biological Background: IL4R Function and Localization

• Interleukin-4 receptor subunit alpha (IL4R, also known as CD124 or IL4RA) is the high-affinity receptor subunit for both interleukin 4 (IL4) and interleukin 13 (IL13). Upon ligand binding, it couples to the JAK1/2/3-STAT6 signaling pathway, driving Th2 differentiation and regulating IgE production, chemokine and mucus secretion in allergic inflammation. Related references: PMID:17030238

• A soluble isoform (sIL4R) is produced by alternative splicing or proteolytic cleavage; it acts as a decoy receptor that inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

• IL4R is expressed as multiple isoforms (e.g., isoform 1 and 2) that are highly expressed in activated T-cells.

• The receptor is localized at the cell membrane and can also be secreted.

• IL4R undergoes post-translational modifications including glycosylation and phosphorylation, and contains disulfide bonds. It is classified as a transmembrane glycoprotein receptor involved in immunity.

• IL4R signaling can also engage insulin receptor substrates (IRS1/IRS2) in certain cell types.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 26-232) of human IL4R, covering the ligand-binding region; this design favors recognition of native receptor forms on the cell surface and soluble isoforms.
• For Western blotting, a band near 90 kDa is anticipated (glycosylation may cause slight shifts); validate in the relevant sample and consider using reducing conditions.
• In immunohistochemistry (paraffin), standard antigen retrieval (e.g., citrate buffer, pH 6.0) is recommended, and endogenous peroxidase blocking should be performed.
• For ELISA applications, this polyclonal antibody can serve as a capture or detection reagent; pairing with a matched monoclonal antibody may improve assay specificity.
• Cross-reactivity with mouse and rat IL4R has been observed; however, perform independent validation in the relevant species and tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for diagnostic manufacturers, laboratories, and research institutes seeking high-quality IVD raw materials. Alongside this anti-IL4R polyclonal antibody, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and assistance with rare target sourcing to advance allergy and immunology diagnostic development. Contact us to discuss your specific requirements and accelerate your project from concept to clinic.