Anti-IL12 Rabbit Polyclonal Antibody for WB, ELISA - P29459 / P29460

Core Product Specifications and Parameters

Biological Background: IL12 (IL-12) Function and Localization

• IL-12 subunit alpha (IL12A) heterodimerizes with IL12B to form IL-12 and with EBI3 to form IL-35, playing distinct immune roles. Related references: PMID:8605935, PMID:8943050
• IL-12 is produced by antigen-presenting cells and regulates T-cell and NK cell responses, inducing IFN-gamma and promoting Th1 differentiation, linking innate and adaptive immunity. Related references: PMID:1673147, PMID:1674604, PMID:8605935
• IL-12 signals through a receptor composed of IL12R1 and IL12R2, leading to JAK-STAT pathway activation, particularly TYK2, JAK2, and STAT4 phosphorylation. Related references: PMID:8943050, PMID:7528775, PMID:7638186
• IL-35 acts as an immune-suppressive cytokine, maintaining liver immune homeostasis and signaling via IL12RB2/gp130 heterodimers or homodimers, requiring STAT1 and STAT4. Related references: PMID:22306691
• Subcellularly, IL12A is secreted and functions as a cytokine.
• Post-translational modifications include disulfide bonds and glycosylation (keywords: Disulfide bond, Glycoprotein).
• Additional keywords: 3D-structure, Direct protein sequencing, Growth factor, Host-virus interaction, Proteomics identification, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen covers both IL12A and IL12B sequences; the antibody may recognize native IL-12 heterodimer and possibly individual subunits. Validate specificity in relevant sample systems.
• For WB, consider that IL12A migrates at ~25 kDa and IL12B at ~37 kDa; reducing conditions may separate the subunits.
• For ELISA, antibody pairs can be designed to detect IL-12 in cell culture supernatants or serum; optimize capture/detection conditions.
• As a rabbit polyclonal antibody, batch-to-batch variability may occur; lot-specific testing is recommended.
• Reported cross-reactivity is human; verify performance in other species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material sourcing by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For IL-12/immune response research, we supply high-quality antibodies and recombinant proteins to support your assay development. Let CamelBio be your reliable partner from discovery to commercial IVD kits.