Anti-IL17RA Rabbit Polyclonal Antibody for WB, ELISA - Q96F46

Core Product Specifications and Parameters

Biological Background: IL17RA Function and Localization

• IL17RA acts as the receptor for the proinflammatory cytokines interleukin-17A (IL-17A) and IL-17F, with a higher binding affinity for IL-17A (PubMed:17911633, PubMed:9367539, PubMed:19838198). Related references: PMID:17911633 PMID:9367539 PMID:19838198
• Upon ligand binding, IL17RA forms a heterodimeric complex with IL17RC, leading to the recruitment of the adapter TRAF3IP2 and activation of NF-kappa-B and MAP kinase pathways via TRAF6, which drives the transcription of cytokines, chemokines, and matrix metalloproteinases (PubMed:16785495, PubMed:17911633, PubMed:18684971). Related references: PMID:16785495 PMID:17911633 PMID:18684971
• IL17RA-mediated signaling is crucial for antimicrobial host defense, particularly by promoting neutrophil activation and recruitment to eliminate extracellular bacteria and fungi (By similarity).
• In secondary lymphoid organs, IL17RA contributes to germinal center formation by regulating B-cell chemotaxis and enhancing somatic hypermutation and plasma cell differentiation (By similarity).
• As an epithelial barrier regulator, IL17RA stimulates the production of antimicrobial beta-defensins (e.g., DEFB1, DEFB103A, DEFB104A) by mucosal epithelial cells, thereby limiting microbial entry (By similarity).
• IL17RA also functions as a receptor for IL-17C in a complex with IL17RE (PubMed:21993848). Related references: PMID:21993848
• During SARS-CoV-2 infection, the viral protein ORF8 can bind to IL17RA, triggering the IL-17 pathway and promoting a pro-inflammatory response.
• Subcellular localization: Primarily localized to the cell membrane, but a soluble secreted form also exists. Tissue expression: Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 33-320) of human IL17RA, so the antibody may recognize the extracellular domain in native and denatured conditions, suitable for WB and ELISA applications.
• For WB, consider using membrane-enriched protein extracts from human, mouse, or rat tissues/cell lines expressing IL17RA. Validate the observed band against the predicted molecular weight (approximately 96 kDa, but glycosylation may cause shifts).
• In ELISA, the antibody can be used for both direct and sandwich formats; however, pairing with a monoclonal capture antibody is advised for sandwich ELISA. Optimize coating concentrations and blocking conditions.
• As a polyclonal antibody, lot-to-lot variability may exist; confirm specificity with a positive control (e.g., IL17RA-overexpressing cells) and negative control (knockdown/knockout) in your experimental setup.
• Cross-reactivity with mouse and rat has been reported, but epitope conservation varies; verify reactivity in your target species by sequence alignment and tissue profiling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio streamlines procurement for immunology and inflammation research. This anti-IL17RA rabbit polyclonal antibody exemplifies our commitment to supporting your IVD development with reliable and well-characterized reagents.