Anti-IL2 Rabbit Polyclonal Antibody for WB, ELISA - P60568

Core Product Specifications and Parameters

Biological Background: IL-2 Function and Localization

• Interleukin-2 (IL-2) is a cytokine primarily produced by activated CD4-positive helper T-cells, with additional production by CD8-positive T-cells and natural killer cells. Related references: PMID:6438535
• IL-2 signals through either a high-affinity trimeric receptor (IL2RA/CD25, IL2RB/CD122, IL2RG/CD132) or a low-affinity dimeric receptor (IL2RB and IL2RG), leading to JAK1/JAK3 activation and STAT5 phosphorylation. Related references: PMID:16293754, PMID:16477002, PMID:7973659, PMID:8580378
• Functions as a potent T-cell growth factor and can enhance the cytolytic activity of natural killer cells. Related references: PMID:6608729
• Promotes proliferation of activated B-cells and subsequent immunoglobulin production. Related references: PMID:6438535
• Plays a critical role in immune tolerance by supporting regulatory T-cell survival and proliferation, and contributes to effector T-cell subset differentiation, including Th1, Th2, Th17, and memory CD8+ T-cells. Related references: PMID:25142963
• IL-2 is a secreted glycoprotein with a molecular weight of approximately 17.6 kDa; it contains a disulfide bond essential for structure.

Experimental Guidance and Technical Tips

• For Western blot, consider using activated T-cell lysates or recombinant IL-2 as a positive control; endogenous IL-2 expression may require stimulation for detection.
• A 12–15% SDS-PAGE gel is suitable for resolving the ~18 kDa IL-2 protein.
• For ELISA, optimize coating and detection antibody concentrations; this polyclonal antibody can serve as capture or detection reagent, but pairing with a monoclonal may improve specificity.
• Validate the antibody in the intended sample type and application to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop provider of IVD raw materials, CamelBio supports diagnostic manufacturers and research laboratories with high-quality antibodies, validated antibody pairs, and bulk ancillary reagents. This anti-IL2 polyclonal antibody is part of our portfolio for immunology and oncology research, enabling reliable detection of this key cytokine. Our sourcing expertise extends to rare targets and custom development to accelerate your assay development from concept to clinic.