Anti-CD215/IL-15R alpha Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13261

Core Product Specifications and Parameters

Biological Background: CD215/IL-15R alpha Function and Localization

• Interleukin-15 receptor subunit alpha (CD215) is a high-affinity receptor for IL-15, mediating both cis and trans signaling to regulate immune cell homeostasis and activation. Related references: PMID:8530383
• In neutrophils, CD215 binds and activates the kinase SYK upon IL-15 stimulation, which is required for IL-15-induced phagocytosis. Related references: PMID:15123770
• Multiple isoforms exist due to alternative splicing; some isoforms do not bind IL-15 and may interfere with signal transduction. Related references: PMID:10480910
• The receptor localizes to various cellular membranes including the plasma membrane, nucleus membrane, endoplasmic reticulum, Golgi apparatus, and cytoplasmic vesicles; a secreted soluble form (sIL-15RA) is also produced.
• Expression is detected in neutrophils at the protein level, and in fetal brain with higher levels in hippocampus and cerebellum; soluble forms are abundant in brain structures.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, as evidenced by UniProt annotation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 167-267 within the extracellular domain of human CD215/IL-15R alpha; this region may be suitable for detecting both membrane-bound and soluble forms. Consider validating detection of the soluble receptor in your assay system.
• For Western blot, the predicted molecular weight is approximately 28 kDa. Glycosylation and isoform diversity may lead to additional bands; validate in the relevant sample system with appropriate controls.
• Immunofluorescence/ICC may reveal membrane and intracellular staining patterns consistent with reported subcellular localizations. Optimization of fixation and permeabilization is recommended.
• This polyclonal antibody may recognize multiple epitopes; for ELISA, consider using recombinant protein standards to ensure specificity and quantify soluble IL-15RA levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD215/IL-15R alpha polyclonal antibody supports immune-oncology and inflammation research, and is part of our comprehensive portfolio of high-quality antibodies and recombinant proteins. Leverage our sourcing expertise to accelerate your assay development with validated antibody pairs, bulk ancillary reagents, and custom solutions.