Anti-IL17F Rabbit Polyclonal Antibody for WB, ELISA - Q96PD4

Core Product Specifications and Parameters

Biological Background: IL17F Function and Localization

• Interleukin-17F (IL-17F), also known as Cytokine ML-1, is a secreted effector cytokine of the innate and adaptive immune system, playing a key role in antimicrobial host defense and tissue integrity maintenance (PubMed:21350122). Related references: PMID:21350122

• IL-17F forms homodimers or heterodimerizes with IL-17A. The IL17A-IL17F heterodimer signals through the IL17RA-IL17RC receptor complex, leading to TRAF6-mediated NF-kappa-B and MAP kinase activation, which drives transcription of pro-inflammatory cytokines, chemokines, and antimicrobial peptides (PubMed:11574464, PubMed:11591732, PubMed:11591768). Related references: PMID:11574464, PMID:11591732, PMID:11591768

• IL-17F homodimer can also signal via an IL17RC homodimeric receptor complex, triggering NF-kappa-B signaling and transcriptional activation of IL33, which is involved in pulmonary allergic responses and sympathetic innervation of peripheral organs (PubMed:32187518). Related references: PMID:32187518

• As a signature cytokine of T-helper 17 (Th17) cells, IL-17F primarily induces neutrophil activation and recruitment to infection and inflammatory sites, contributing to defense against extracellular bacteria and fungi.

• IL-17F stimulates the production of antimicrobial beta-defensins (DEFB1, DEFB103A, DEFB104A) by mucosal epithelial cells, limiting microbial entry, and regulates intestinal microbiota composition by controlling commensal Firmicutes and Bacteroidetes.

• IL-17F is expressed in activated T-helper 1 and T-helper 2 cells, basophils, and mast cells, and is secreted into the extracellular space.

• Key structural features include a signal peptide, disulfide bonds, and glycosylation; the mature protein has a molecular weight of approximately 18 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 31–163 of human IL17F (NP_443104.1), covering the mature secreted form. This design targets the extracellular functional domain.
• For Western blot, consider the predicted protein weight of ~18 kDa under reducing conditions. Verify band specificity in relevant positive control lysates (e.g., activated T-cell extracts).
• For ELISA, validate the antibody in direct or sandwich formats with recombinant IL17F protein as standard.
• Given cross-reactivity with human and mouse IL17F, the antibody may be used in comparative studies; confirm performance in each species-specific sample system.
• Optimize antibody concentrations for each application and sample type to account for batch-to-batch variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, specializing in cytokines and immune-related targets. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories in developing immunoassays for interleukin targets like IL17F. Our supply network streamlines procurement from concept to clinic, ensuring reliable access to rare and routine raw materials.