Anti-IL17RD Polyclonal Antibody for WB and ELISA - Q8NFM7

Core Product Specifications and Parameters

Biological Background: IL17RD Function and Localization

• IL17RD, also known as Sef homolog (hSef) or IL17Rhom, is a transmembrane receptor belonging to the interleukin-17 receptor family. Alternative splicing produces multiple isoforms (e.g., isoform 1 and isoform 4).
• It functions as a feedback inhibitor of fibroblast growth factor (FGF)-mediated Ras-MAPK signaling, suppressing ERK activation by blocking nuclear translocation of phosphorylated ERK. Related references: PMID:12807873 PMID:12958313 PMID:15239952
• May also mediate JNK activation and apoptosis, inhibit FGF-induced FGFR1 tyrosine phosphorylation, and suppress TGFB-induced epithelial-to-mesenchymal transition.
• Localizes to the Golgi apparatus membrane, cell membrane, and cytoplasm.
• Highly expressed in vascularized tissues (kidney, colon, heart) and ductal epithelial cells; isoform 1 is abundant in fetal and adult brain, pituitary, and reproductive tissues; isoform 4 is specifically enriched in pituitary and fetal brain.
• Contains disease-associated variants linked to hypogonadotropic hypogonadism and Kallmann syndrome. The protein is glycosylated and features a signal peptide and transmembrane domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 150–300 of human IL17RD (NP_060033.3), a region conserved in mouse and rat, supporting the reported cross-reactivity. For Western blot, positive control lysates from kidney, colon, or heart tissues are recommended based on tissue expression data.
• The predicted molecular weight is ~82 kDa (UniProt: 82.4 kDa), but post-translational modifications such as glycosylation may cause slight migration shifts. Optimize blocking and antibody dilution for each sample type.
• For ELISA, consider using recombinant IL17RD extracellular domain fragments as capture antigen. Validate the assay with known positive/negative samples in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high-quality IVD raw materials. This IL17RD antibody suits investigations into growth factor signaling, receptor trafficking, and developmental disorders. Beyond single reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials—streamlining your workflow from discovery to diagnostic development.