Anti-GATM Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P50440

Core Product Specifications and Parameters

Biological Background: GATM Function and Localization

• GATM, also known as glycine amidinotransferase, L-arginine:glycine amidinotransferase, or transamidinase, is a mitochondrial enzyme encoded by the GATM gene in humans.
• It functions as a transamidinase, catalyzing the transfer of the amidino group from L-arginine to acceptor metabolites such as glycine, beta-alanine, GABA, and taurine, generating guanidino derivatives.
  Related references:
  PMID:16820567 PMID:27233232 PMID:36543883
• In creatine biosynthesis, GATM catalyzes the rate-limiting step: the conversion of L-arginine and glycine to guanidinoacetate, which is subsequently methylated by GAMT to form creatine. Creatine serves as an essential source for ATP regeneration, particularly in high‑energy‑demand tissues like skeletal muscle, heart, and brain.
  Related references:
  PMID:27233232 PMID:36543883 PMID:3800397
• The protein localizes primarily to the mitochondrial inner membrane, with additional cytoplasmic presence.
• Tissue expression is broad, with the highest levels observed in the kidney; it is also detected in brain, heart, liver, lung, salivary gland, and skeletal muscle. Biallelic expression is found in placenta and fetal tissues.
• Post‑translational modifications include acetylation and phosphorylation, and alternative splicing yields multiple isoforms.
• Mutations in GATM are associated with disease variants (e.g., arginine:glycine amidinotransferase deficiency), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 38‑423 of human GATM; thus, it may recognize both full‑length and truncated forms. Validate in the relevant sample system.
• For Western blot, a band around 48 kDa is expected. Consider using reducing conditions and include positive control lysates from human, mouse, or rat tissues with known GATM expression (e.g., kidney or skeletal muscle).
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), optimize antigen retrieval and blocking steps, and confirm specificity with appropriate controls.
• ELISA applications may require antibody titration and pairing with suitable detection reagents. Cross‑reactivity testing across species is recommended when using non‑human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including the anti‑GATM rabbit monoclonal antibody for mitochondrial metabolism and creatine disorder research. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets—positioning us as your procurement partner from concept to clinical application.