Anti-TFAM Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - Q00059

Core Product Specifications and Parameters

Biological Background: TFAM Function and Localization

• TFAM (Transcription Factor A, Mitochondrial) is a member of the HMGB protein family and exists in several isoforms due to alternative splicing.
• Binds to the mitochondrial light strand promoter and functions as a key transcriptional activator. Related references: PMID:29445193 PMID:32183942
• Forms the mitochondrial transcription initiation complex with TFB2M and POLRMT; recruits POLRMT to promoters and induces structural changes enabling transcription. Related references: PMID:29149603 PMID:20410300
• Enhances mitochondrial RNA polymerase promoter recognition and promotes transcription initiation from HSP1 and light strand promoters; possesses DNA unwinding activity. Related references: PMID:22037172
• Bends the light strand promoter DNA into a U-turn shape via its HMG boxes, facilitating transcription initiation. Related references: PMID:1737790
• Required for maintenance of normal mitochondrial DNA levels; may also organize and compact mtDNA. Related references: PMID:19304746 PMID:22841477 PMID:22037171
• Localizes to the mitochondrial matrix and nucleoids; undergoes phosphorylation.
• Pathogenic mutations in TFAM cause mitochondrial DNA depletion syndrome 15 (MTDPS15), a primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of human TFAM; this region may be conserved in mouse and rat, consistent with cross-reactivity data.
• For Western blot (WB), the predicted molecular weight is approximately 29 kDa; consider using whole-cell or mitochondrial extracts for optimal detection.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), standard antigen retrieval and permeabilization protocols are recommended; validate in appropriate positive and negative control tissues.
• For ELISA, the antibody may be used as a capture or detection reagent; titrate to determine optimal working concentration.
• Always perform pilot experiments to confirm specificity and performance in your specific assay system and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. This anti-TFAM rabbit monoclonal antibody supports research and assay development in mitochondrial biology, transcription regulation, and related disease areas. With end-to-end support from concept to clinic, CamelBio accelerates your diagnostic development through tailored sourcing solutions and technical consultation.