GABA transaminase (ABAT) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P80404

Core Product Specifications and Parameters

Biological Background: GABA transaminase (ABAT) Function and Localization

• ABAT encodes the mitochondrial enzyme 4-aminobutyrate aminotransferase, also known as GABA aminotransferase (GABA-AT), (S)-3-amino-2-methylpropionate transaminase, and L-AIBAT (UniProt: P80404).

• Catalyzes the conversion of gamma-aminobutyrate (GABA) to succinate semialdehyde, a key step in neurotransmitter degradation; also acts on L-beta-aminoisobutyrate to produce methylmalonate semialdehyde. Related references: PMID:10407778 PMID:15528998

• Can utilize delta-aminovalerate and beta-alanine as substrates (By similarity).

• Localizes to the mitochondrial matrix, imported via an N-terminal transit peptide.

• Contains a pyridoxal phosphate cofactor and an iron-sulfur cluster essential for catalytic activity (UniProt keywords: Aminotransferase, Iron, Iron-sulfur, Pyridoxal phosphate, Transferase).

• Post-translational modifications include acetylation and disulfide bond formation (UniProt keywords: Acetylation, Disulfide bond).

• Tissue expression: highest in liver, followed by pancreas, brain, kidney, heart, and placenta.

• Listed as a disease variant; mutations in ABAT are linked to neurological disorders (UniProt keyword: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-100), which includes the mitochondrial transit peptide; the antibody may detect both precursor and mature forms. Consider this banding pattern in WB.

• For Western blotting, the predicted molecular weight is ~56 kDa. Use an appropriate loading control and validate specificity with positive/negative lysates.

• In IHC-P, antigen retrieval may be necessary; include isotype controls and verify staining patterns relative to known tissue expression profiles.

• For ELISA, the antibody can serve as a capture or detection reagent; titrate under assay-specific conditions and confirm sensitivity with recombinant antigen.

• Cross-reactivity with mouse and rat is indicated; when using non-human samples, validate performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects focused on GABA metabolism, mitochondrial dysfunction, or neurotransmitter degradation, we provide comprehensive sourcing support from early-stage discovery to clinical application. Leverage our expertise to accelerate your assay development with reliable, customizable raw materials.