Anti-GAB1 Rabbit Monoclonal Antibody for WB, ELISA - Q13480

Core Product Specifications and Parameters

Biological Background: GAB1 Function and Localization

• GAB1 (GRB2-associated-binding protein 1) is an adaptor protein also known as GRB2-associated binder 1 or Growth factor receptor bound protein 2-associated protein 1.
• It transduces signals from activated receptor tyrosine kinases such as FGFR1, EGFR, and the insulin receptor (INSR).
• GAB1 is critically involved in the MET/HGF-signaling pathway. Related references: PMID:29408807
• Alternative splicing of GAB1 yields multiple isoforms, and the protein is regulated by post-translational modifications including acetylation and phosphorylation.
• Mutations in GAB1 are linked to autosomal recessive non-syndromic deafness (DFNB26).
• The calculated molecular mass of GAB1 is 76.6 kDa, and it migrates at 76–80 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 594–694) of human GAB1; this region may contain conserved epitopes explaining the observed mouse cross‑reactivity.
• For western blot, consider a starting dilution of 1:500–1:2000 and validate in a lysate known to express the target.
• ELISA can be optimized for detection of recombinant or endogenous GAB1; pilot experiments with different coating conditions are recommended.
• Confirm the detected band matches the expected 76–80 kDa range, and include positive/negative controls to verify specificity.
• Multiple isoforms exist due to alternative splicing; the antibody may recognize several if the epitope is shared.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this GAB1 rabbit monoclonal antibody as part of a complete portfolio of validated antibodies and ancillary reagents for signal transduction research. Our one‑stop IVD raw‑material sourcing model ensures seamless procurement—from optimized antibody pairs and bulk monoclonal/polyclonal antibodies to rare‑target materials—supporting your development from concept to clinic.