Anti-Transglutaminase 2 (TGM2) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P21980

Core Product Specifications and Parameters

Biological Background: Transglutaminase 2 (TGM2) Function and Localization

• TGM2 is a calcium-dependent enzyme that catalyzes the formation of isopeptide bonds between glutamine and lysine residues, crucial for extracellular matrix stabilization and apoptosis. Related references: PMID:23941696, PMID:9252372, PMID:7935379, PMID:12506096
• In the nucleus, TGM2 mediates serotonylation and dopaminylation of histone H3, directly regulating chromatin organization and transcriptional plasticity in neurons. Related references: PMID:30867594, PMID:32273471
• TGM2 exhibits protein deamidase activity, converting specific glutamine residues to glutamate, a mechanism implicated in celiac disease via gliadin modification. Related references: PMID:20547769, PMID:9623982
• Its cross-linking activity is inhibited by GTP; Ca2+ binding relieves this inhibition, and the enzyme can also function as a GTP-binding signal transducer in adrenergic receptor pathways. Related references: PMID:18092889, PMID:7592956, PMID:7649299, PMID:8943303
• TGM2 localizes to the cytoplasm, nucleus, extracellular matrix, and mitochondria, reflecting its multifunctional roles.
• Subject to acetylation, phosphorylation, S-nitrosylation, and proteolytic processing, which modulate its enzymatic and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 438–687), which includes the catalytic core; the antibody may recognize both full-length and certain cleaved forms of TGM2.
• For WB, a prominent band at ~77 kDa is expected under reducing conditions; consider using fresh samples and protease inhibitors to prevent degradation.
• In IHC-P, antigen retrieval with citrate buffer (pH 6.0) may improve staining; validate the antibody in human tissue arrays or appropriate positive controls.
• For IF/ICC, fixation with 4% PFA and permeabilization with 0.1% Triton X-100 are typical starting points; co-staining with organelle markers can confirm localization.
• ELISA applications may benefit from using a matched antibody pair or direct detection; consider checkerboard titrations to determine optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material partner supporting diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—all tailored to accelerate your TGM2-related assay development. Whether you are building protein cross-linking, apoptosis, or chromatin modification assays, our team helps source rare targets and provides technical consulting from concept to clinic. Count on CamelBio as your B2B bridge to streamlined, one-stop IVD raw-material procurement.