KD Validated Anti-GM130 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q08379

Core Product Specifications and Parameters

Biological Background: GM130 Function and Localization

• GM130 (GOLGA2) is a 130 kDa cis-Golgi matrix protein also known as golgin-95, belonging to the golgin family of coiled-coil proteins.
• Acts as a peripheral membrane skeleton maintaining the structure of the Golgi apparatus and facilitating vesicle tethering to the Golgi membrane. Related references: PMID:16489344
• Together with p115/USO1 and STX5, mediates vesicle tethering and fusion at the cis-Golgi network, essential for the stacked and interconnected Golgi architecture.
• Required for normal protein transport from the endoplasmic reticulum to the Golgi and cell membrane (By similarity).
• Central to mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at mitosis onset disrupts p115/USO1 interaction, blocking COPI vesicle tethering and halting Golgi transport.
• Plays a critical role in spindle pole assembly and centrosome organization by activating TPX2 through importin-alpha sequestration, thereby nucleating microtubules around the Golgi; also regulates meiotic spindle assembly. Related references: PMID:19242490, PMID:26165940
• Required for Golgi ribbon formation and proper glycosylation of membrane and secretory proteins. Related references: PMID:16489344, PMID:17314401
• Localizes to the cis-Golgi network membrane, endoplasmic reticulum-Golgi intermediate compartment, and mitotic spindle poles; subject to methylation and alternative splicing; mutations are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–300 of human GM130 (NP_004477.3), targeting a region upstream of the coiled-coil domains; this may recognize both full-length and any N-terminal isoforms.
• Recommended applications include Western blot (detected at ~130 kDa), immunofluorescence/immunocytochemistry (IF/ICC), paraffin-embedded immunohistochemistry (IHC-P), and ELISA, as supported by the supplier.
• Species cross-reactivity with human, mouse, and rat has been reported; validate in your experimental model.
• For immunofluorescence, GM130 serves as a well-established cis-Golgi marker; use appropriate fixation and permeabilization protocols to visualize Golgi morphology.
• Mitotic or stress-induced Golgi fragmentation may alter GM130 staining pattern; consider co-staining with other organelle markers.
• For ELISA, the antibody can be used as a detection or capture reagent; optimization of coating conditions and antibody concentration is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting your GM130/Golgi-related assays from development to commercialization. In addition to validated GM130 antibodies, we supply matched antibody pairs, ancillary reagents, and custom rare-target raw materials to streamline your workflow.