Anti-CD146 Rabbit Monoclonal Antibody for WB, IHC-P, FC, ELISA - Q8R2Y2

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• CD146, also designated MUC18, Gicerin, or Melanoma cell adhesion molecule (MCAM), is a cell surface glycoprotein encoded by the Mcam gene (mouse GeneID: 84004; human GeneID: 4162).
• As a member of the immunoglobulin superfamily, CD146 contains multiple Ig-like domains, a transmembrane helix, and a cytoplasmic tail, functioning as a key adhesion molecule.
• CD146 mediates homophilic cell-cell adhesion and is critical for maintaining endothelial monolayer integrity at intercellular junctions in vascular tissue.
• Its expression on melanoma cells facilitates interactions with vascular elements, potentially promoting hematogenous tumor spread.
• During embryonic development, CD146 may act as an adhesion molecule active in neural crest cells (By similarity).
• Upon engagement, CD146 acts as a surface receptor triggering tyrosine phosphorylation of FYN and FAK1 and a transient rise in intracellular calcium (By similarity).
• Subcellular localization is primarily at the membrane, consistent with its transmembrane adhesion and signaling roles.
• Post‑translational modifications include disulfide bond formation, glycosylation, and phosphorylation, as indicated by UniProt keywords; alternative splicing is also reported.
• Expression is notably detected in melanoma cell lines, underscoring its relevance in oncology and metastasis research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24–563 of mouse CD146 (extracellular region), suggesting the antibody may recognize full‑length and certain extracellular splice variants.
• For Western blotting, a band around 71 kDa is expected, though glycosylation may cause a higher apparent molecular weight; validate in your specific lysate.
• In immunohistochemistry (paraffin), consider antigen retrieval to optimize epitope exposure; in flow cytometry, use appropriate isotype controls.
• As cross‑reactivity is reported for human, mouse, and rat, this antibody can be employed in multi‑species studies, but always verify performance in your experimental setup.
• For ELISA and other applications, inclusion of positive and negative controls is recommended to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one‑stop partner for diagnostic manufacturers, laboratories, and research institutes seeking IVD raw materials. For CD146 and related cell adhesion targets, our platform provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and reliable sourcing of rare‑target raw materials. From concept to clinic, CamelBio streamlines your supply chain with quality and consistency, enabling accelerated assay development.