Anti-CDC40 Rabbit Monoclonal Antibody (PRP17/PRPF17) for WB, IHC-P, ELISA - O60508

Core Product Specifications and Parameters

Biological Background: CDC40 Function and Localization

• CDC40, also known as Pre-mRNA-processing factor 17, PRP17 homolog, Cell division cycle 40 homolog, EH-binding protein 3 (EHB3), and PCH15, is a conserved spliceosomal protein.
• It is required for pre-mRNA splicing as a component of the activated spliceosome and plays an essential role in embryonic brain development; this developmental function is independent of proline isomerization activity. Related references: PMID:33220177
• The protein localizes to the nucleus and is enriched in nuclear speckles, consistent with its role in pre-mRNA processing.
• CDC40 is a phosphoprotein containing WD repeat motifs that facilitate protein-protein interactions.
• Mutations in CDC40 are associated with intellectual disability, highlighting its neurological importance.
• It belongs to the spliceosome and WD repeat protein families and is part of the reference proteome.

Experimental Guidance and Technical Tips

• Based on the immunogen design against the N-terminal region (amino acids 1–100), the antibody is expected to recognize all known isoforms. Validate specificity in your target tissue or cell line.
• For Western blot, a specific band around 66 kDa should be detected. Use a suitable loading control (e.g., GAPDH) and verify molecular weight markers.
• IHC-P applications may benefit from systematic optimization of antigen retrieval (heat-induced or enzymatic) and antibody dilution.
• Given the predicted cross-reactivity with mouse and rat, the antibody can be used in these species; however, confirm performance in your specific experimental system.
• ELISA can be utilized with the immunizing peptide as a positive control to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a streamlined sourcing experience for IVD raw materials. This Anti-CDC40 monoclonal antibody is part of our growing portfolio of validated reagents for spliceosome and neurological disorder research. From bulk antibody orders to custom rare-target procurement, CamelBio supports your entire development pipeline from concept to clinic.