Anti-CD14 Monoclonal Antibody for IF/ICC, IHC, ELISA - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 functions as a coreceptor for bacterial lipopolysaccharide (LPS), initiating the innate immune response to gram-negative bacteria.
  Related references: PMID:1698311, PMID:23264655

• In collaboration with LBP, CD14 binds monomeric LPS and transfers it to the LY96/TLR4 complex, triggering MyD88-dependent NF-kB activation and pro-inflammatory cytokine secretion.
  Related references: PMID:20133493, PMID:22265692, PMID:23264655, PMID:8612135

• CD14 also serves as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides, respectively, facilitating signaling from the cell surface and subsequent trafficking to the Golgi.
  Related references: PMID:16880211

• Beyond LPS, CD14 binds electronegative LDL (LDL(-)) and mediates cytokine release induced by modified lipoproteins.
  Related references: PMID:23880187

• CD14 is localized to the cell membrane, membrane rafts, and Golgi apparatus; a soluble form is also secreted, expanding its functional range.

• Tissue expression is predominantly on monocytes and macrophages, with weak detection on granulocytes.
  Related references: PMID:1698311

• The protein is heavily glycosylated and anchored to the membrane via a GPI linkage; it contains leucine-rich repeats and disulfide bonds critical for its structure and ligand recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 250-350 of human CD14; this region may be used for specific peptide blocking experiments to confirm antibody specificity.
• For immunofluorescence (IF/ICC and IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization protocols since CD14 is a membrane-anchored protein; antigen retrieval may be unnecessary for cell surface targets.
• In ELISA applications, consider using the antibody as a capture or detection reagent; validate its performance with recombinant CD14 or native samples from monocytic cell lines.
• Reported cross-reactivity is limited to human; if your experiments involve other species, confirm sequence homology of the epitope region and test cross-reactivity empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials for innate immunity and inflammation research. We supply validated antibody pairs, optimized monoclonal antibodies like this anti-CD14 clone, bulk ancillary reagents, and can assist with rare target sourcing. Let CamelBio be your partner from concept to clinic, streamlining your diagnostic development workflow.