Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for Flow Cytometry - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14 is a myeloid cell-specific leucine-rich glycoprotein, also known as monocyte differentiation antigen CD14 or My23 antigen.
• CD14 functions as a key coreceptor for bacterial lipopolysaccharide (LPS), mediating innate immune recognition and response. Related references: PMID:1698311, PMID:23264655
• In concert with LBP, CD14 binds monomeric LPS and delivers it to the LY96/TLR4 complex, thereby driving NF-kappa-B activation and pro-inflammatory cytokine secretion via MyD88-, TIRAP- and TRAF6-dependent pathways. Related references: PMID:20133493, PMID:22265692, PMID:23264655, PMID:8612135
• Additionally, CD14 acts as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers, recognizing diacylated and triacylated lipopeptides, respectively, and triggering lipid-raft-dependent signaling. Related references: PMID:16880211
• CD14 also binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-), implicating it in atherosclerosis-related inflammation. Related references: PMID:23880187
• At the protein level, CD14 is strongly detected on the surface of monocytes and weakly on granulocytes; it is also expressed by most tissue macrophages. Related references: PMID:1698311
• Subcellularly, CD14 is located at the cell membrane, enriched in membrane rafts, and also found in the Golgi apparatus; a secreted form exists as well.
• The protein is a GPI-anchored glycoprotein with multiple leucine-rich repeats and disulfide bonds, features that are critical for its function.

Experimental Guidance and Technical Tips

• For flow cytometry staining, titrate the -conjugated antibody using human peripheral blood mononuclear cells (PBMCs) or cynomolgus monkey leukocytes to determine the optimal dilution.
• Since CD14 is a GPI-anchored membrane protein, use a cell surface staining protocol; avoid permeabilization unless intracellular staining is intended, as this may disrupt raft-localized complexes.
• Consider co-staining with monocyte/macrophage markers (e.g., CD16, CD163) and viability dyes to accurately gate on monocyte populations.
• Verify cross-reactivity in cynomolgus samples if advancing to non-human primate studies; the antibody’s reported reactivity with cynomolgus allows translational research.
• As CD14 can be shed as a soluble form, avoid excessive washing steps that might remove loosely bound antibody; use 0.5% BSA/PBS buffer for staining.
• For data analysis, use appropriate compensation controls when using , and gate on FSC/SSC to identify monocyte/macrophage populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and research laboratories with access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing. Our Rabbit anti-Human/Monkey CD14 monoclonal antibody reflects our commitment to supporting innate immunity and inflammation research, from biomarker discovery to clinical assay development. Let CamelBio accelerate your path from concept to clinic with reliable, high-quality raw material supply solutions.