Anti-CD146/MCAM Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P43121

Core Product Specifications and Parameters

Biological Background: CD146/MCAM Function and Localization

• CD146/MCAM (Cell surface glycoprotein MUC18) is a single-pass type I membrane glycoprotein belonging to the immunoglobulin superfamily.
• It mediates homophilic cell adhesion and is crucial for maintaining endothelial monolayer integrity at intercellular junctions.
• In cancer, CD146 expression facilitates hematogenous metastasis by promoting melanoma cell interaction with the vascular endothelium.
• Functions as a surface receptor, activating intracellular signaling cascades including tyrosine phosphorylation of FYN and PTK2/FAK1, and inducing calcium mobilization.
• During embryonic development, CD146 acts as an adhesion molecule guiding neural crest cell migration.
• Alternatively spliced isoforms exist, and the protein is subject to disulfide bond formation, N-glycosylation, and phosphorylation.
• Tissue expression is predominantly in vascular endothelial cells and smooth muscle, with dramatically upregulated levels in metastatic melanoma lesions.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 26–350), suggesting the antibody recognizes an extracellular epitope, suitable for detecting cell surface CD146 by IF/ICC and potentially capturing soluble forms in ELISA.
• For Western blot, validate in human endothelial (e.g., HUVEC) or melanoma cell lines; expect a band at approximately 72 kDa under reducing conditions.
• In immunofluorescence, optimize cell fixation and permeabilization to preserve membrane localization; CD146 typically localizes to the plasma membrane.
• Due to its role in tumor metastasis, CD146 may serve as a biomarker; consider pairing this antibody with appropriate standards in ELISA assays for quantification in patient-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop access to high-quality IVD raw materials, supporting projects from concept to clinic. Our anti-CD146/MCAM monoclonal antibody is ideal for vascular biology and oncology research, and we are positioned to supply validated antibody pairs, bulk ancillary reagents, and even rare-target raw-material sourcing. Whether you need optimized monoclonals, polyclonals, or customized solutions, CamelBio bridges your procurement needs with reliable supply chains.