PE Rabbit Anti-Mouse CD14 Monoclonal Antibody for Flow Cytometry - P10810

Core Product Specifications and Parameters

Biological Background: Monocyte differentiation antigen CD14 Function and Localization

• CD14 (Monocyte differentiation antigen CD14), also known as myeloid cell-specific leucine-rich glycoprotein, is a GPI-anchored glycoprotein encoded by the Cd14 gene.
• Acts as a coreceptor for bacterial lipopolysaccharide (LPS) by partnering with LBP to deliver LPS to the LY96/TLR4 complex, thereby mediating the innate immune response (PubMed:16148141). Related references: PMID:16148141
• Triggers intracellular signaling via MyD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and inflammatory response (PubMed:15895089, PubMed:8612135). Related references: PMID:15895089 PMID:8612135
• Serves as an accessory receptor for M. tuberculosis lipoproteins LprA, LprG, and LpqH in conjunction with TLR2/TLR1, modulating antigen-presenting cell functions (PubMed:19362712). Related references: PMID:19362712
• Binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-) (By similarity).
• Localizes to the cell membrane, raft regions, and Golgi apparatus; a soluble secreted form is also produced.
• Expressed on peritoneal macrophages, lung alveolar macrophages, and dendritic macrophages (PubMed:8612135, PubMed:19362712).
• Key structural features include leucine-rich repeats and a GPI-anchor; involved in innate immunity, inflammatory response, and defense against bacterial pathogens.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 18–345 of the mouse CD14 extracellular domain. This rabbit monoclonal antibody is supplied as a PE conjugate for direct flow cytometry.
• For FC experiments, titrate the antibody to determine the optimal staining concentration using relevant murine macrophage or monocyte cell lines (e.g., RAW 264.7) or primary mouse peritoneal macrophages.
• Because CD14 exists in both membrane-bound (GPI-anchored) and soluble forms, use non‑permeabilizing staining conditions to preserve surface expression unless intracellular detection is intended.
• Validate specificity in mouse samples; cross‑reactivity with other species is not indicated by the provided data.
• Include appropriate isotype controls and Fc receptor blocking reagents to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive, one‑stop sourcing solution for IVD raw materials, from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. This PE anti‑mouse CD14 antibody supports innate immunity and inflammation research, and we can also source matched antibody pairs, optimized clones, and rare‑target raw materials. Partner with us to efficiently move your diagnostic or research project from concept to clinic.