Anti-CD24 Rabbit Monoclonal Antibody for WB, IF, FC, ELISA - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24, also known as heat stable antigen (HSA), nectadrin, and lymphocyte antigen 52 (Ly-52), is a signal transducer protein expressed on lymphoid, myeloid, and erythroid cells.
• It plays critical roles in cell differentiation, particularly in early thymocyte development, and modulates B-cell activation responses.
• In association with SIGLEC10, CD24 selectively suppresses immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Related references: PMID:19264983
• CD24 is involved in the control of autoimmunity. Related references: PMID:20200274
• The protein is anchored to the cell membrane via a GPI anchor and has a signal peptide; the mature polypeptide is approximately 8 kDa (7.8 kDa calculated from sequence).
• CD24 is a glycoprotein involved in innate immunity, and its GPI-anchored structure facilitates signaling through release of second messengers.
• It is highly expressed on hematopoietic cells, consistent with its role in immune regulation.

Experimental Guidance and Technical Tips

• For Western blotting, CD24 has a predicted molecular weight of ~8 kDa; use high-percentage gels (e.g., 15% SDS-PAGE) and low-range molecular weight markers for accurate detection.
• In immunofluorescence applications (IF/ICC, IF-P), CD24 localizes to the cell membrane; primary antibody dilution and permeabilization conditions should be optimized for the sample type.
• For flow cytometry, use an anti-rabbit IgG secondary antibody and ensure proper cell surface staining with live, unfixed cells to preserve GPI-anchored epitopes.
• For ELISA, the immunogen sequence (amino acids 27–53) corresponds to the N-terminal extracellular domain of mature CD24; consider using recombinant CD24 as a positive control.
• Since this antibody is rabbit monoclonal and targets mouse CD24, use species-matched secondary antibodies to avoid cross-reactivity with endogenous mouse immunoglobulins in mouse tissue or cell samples.
• Validate antibody specificity using appropriate controls such as CD24 knockout cell lines or blocking peptides, especially when interpreting results from complex tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized rare-target raw-material procurement. For researchers studying CD24 in immune regulation, autoimmunity, or inflammatory diseases, we provide reliable access to this key reagent and support your journey from concept to clinic with integrated technical services and consulting.