Anti-Mouse CD146 Monoclonal Antibody () for FC - Q8R2Y2

Core Product Specifications and Parameters

Biological Background: CD146 Function and Localization

• CD146, also known as MUC18 or gicerin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. It contains immunoglobulin-like domains, a transmembrane helix, and undergoes alternative splicing.
• It functions as an adhesion molecule, mediating cell-cell interactions and contributing to the cohesion of the endothelial monolayer at intercellular junctions in vascular tissue.
• CD146 expression may facilitate melanoma cell interaction with the vascular system, potentially enhancing hematogenous tumor spread.
• In embryonic development, CD146 is active in neural crest cells, suggesting a role in cell adhesion during morphogenesis.
• Binding of ligands to CD146 triggers intracellular signaling, including tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in intracellular calcium concentration (By similarity).
• The protein is localized to the membrane and is detected in melanoma cell lines.
• Post-translational modifications include phosphorylation and N-glycosylation; it also contains disulfide bonds that stabilize its immunoglobulin domains.

Experimental Guidance and Technical Tips

• As the antibody is directly conjugated to , flow cytometry can be performed without the need for secondary antibodies, reducing non-specific binding and saving time.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and instrument settings.
• Validate staining in a relevant mouse cell line or tissue where CD146 expression is expected, such as melanoma cells or endothelial cells.
• Use appropriate isotype controls and viability dyes to ensure specific detection and accurate data interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from research-grade antibodies to bulk ancillary reagents. With this conjugated anti-mouse CD146 monoclonal antibody, researchers studying melanoma and endothelial biology can conveniently incorporate flow cytometric detection into their workflow. As part of our commitment to supporting diagnostic development, we also specialize in sourcing rare-target raw materials and providing technical consultation from concept to clinic.