Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for Flow Cytometry - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14, also designated My23 antigen, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein and a member of the leucine-rich repeat (LRR) family.
• Functions as a coreceptor for bacterial lipopolysaccharide (LPS). In concert with LBP, CD14 captures monomeric LPS and transfers it to the LY96/TLR4 complex, triggering innate immune signaling. Related references: PMID:1698311 PMID:23264655
• Downstream signaling requires the adaptors MyD88, TIRAP, and TRAF6, ultimately leading to NF-kappa-B activation and the secretion of proinflammatory cytokines. Related references: PMID:8612135
• CD14 also functions as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers, recognizing diacylated and triacylated lipopeptides respectively. These receptor clusters signal from the cell surface before being internalized to the Golgi via a lipid-raft-dependent route. Related references: PMID:16880211
• Additionally, CD14 binds electronegative LDL (LDL(-)) and mediates the cytokine release elicited by this modified lipoprotein. Related references: PMID:23880187
• The protein is predominantly located on the plasma membrane, enriched in membrane rafts, and traffics to the Golgi; a soluble form is also shed into the extracellular milieu.
• CD14 is highly expressed on the surface of monocytes and most tissue macrophages, with lower levels detectable on granulocytes. Related references: PMID:1698311

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 20-352), targeting a region critical for ligand binding; verify reactivity with both membrane-bound and soluble CD14 in the relevant sample system.
• As an conjugate, use appropriate laser and filter settings for flow cytometry (e.g., 488 nm excitation, 500–550 nm emission). Titrate the antibody to optimize signal-to-noise ratio on monocyte/macrophage populations.
• Confirm expression in positive controls such as human PBMCs or isolated monocytes; cynomolgus cross-reactivity allows for non-human primate translational studies.
• For intracellular staining or detection of the Golgi-localized pool, consider permeabilization steps after surface labeling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting the full development pipeline from concept to clinic. Our portfolio includes validated monoclonal antibodies, antibody pairs, and ancillary reagents for targets like CD14—essential for innate immunity and inflammatory disease diagnostics. Whether you require bulk quantities, custom conjugation, or rare-target raw materials, CamelBio simplifies procurement to accelerate your assay development.