Phospho-Lyn-Y397 Rabbit mAb - P07948

Core Product Specifications and Parameters

Biological Background: Lyn Function and Localization

• Lyn is a non-receptor tyrosine-protein kinase belonging to the Src family, encoded by the LYN gene (also known as JTK8). It exists as two main isoforms (p56Lyn and p53Lyn) through alternative splicing.
• Lyn transmits signals from diverse cell surface receptors, playing a central role in innate and adaptive immunity, hematopoiesis, growth factor/cytokine responses, and integrin signaling.
• In B cells, Lyn is essential for both initiation and termination of B-cell receptor signaling, regulating differentiation, proliferation, survival, and apoptosis, which is crucial for immune self-tolerance.
• Lyn mediates inflammatory responses to bacterial lipopolysaccharide (LPS) and acts downstream of TLR2/TLR4, facilitating TLR4-TLR6 heterodimerization and signal initiation.
• Lyn is involved in the regulation of endothelial activation, neutrophil adhesion, and transendothelial migration, critical steps in the inflammatory response. Related references: PMID:36932076
• Kinase activity leads to phosphorylation of adaptor proteins BCAR1/CAS and NEDD9/HEF1, contributing to integrin signaling and cell adhesion dynamics. Related references: PMID:9020138
• Subcellularly, Lyn localizes to the cell membrane, cytoplasm, perinuclear region, Golgi apparatus, and nucleus, reflecting its multiple functional compartments.
• The protein is widely expressed in various tissues, including hematopoietic and neuronal cells, with prominent expression in monocytes and primary neuroblastoma tumors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Tyr396 of human Lyn (corresponding to Tyr397 in certain isoforms); validate the phospho-specificity and consider isoform-appropriate detection in your experimental system.
• For western blotting, expected bands are at approximately 56 kDa (p53Lyn) and 59 kDa (p56Lyn). Use phosphatase inhibitors during cell/tissue lysis to preserve phosphorylation.
• Demonstrated cross-reactivity with mouse makes this antibody suitable for mouse model studies; verify reactivity with your specific mouse strain and sample type.
• In ELISA applications, optimize the peptide coating concentration and use non-phosphorylated peptide controls to confirm phospho-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, connecting diagnostic manufacturers and labs with validated antibodies, optimized reagents, and bulk ancillary supplies. For researchers focused on tyrosine kinase signaling and immune modulation, we ensure consistent lot-to-lot performance and can source rare-target raw materials, supporting your projects from concept to clinic.