Phospho-FAK-Y397 Rabbit mAb for WB and ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• FAK (Focal Adhesion Kinase 1) is a non-receptor protein-tyrosine kinase encoded by the PTK2 gene, also referred to as p125FAK or pp125FAK. It serves as a central mediator of integrin signaling, regulating cell adhesion, migration, proliferation, and survival.
• Upon activation at focal adhesions, FAK autophosphorylates at tyrosine 397, creating a high-affinity docking site for SRC family kinases and initiating downstream signaling cascades critical for cytoskeletal remodeling.
• FAK integrates signals from diverse receptors, including integrins, growth factor receptors, GPCRs, EPHA2, and netrin receptors, coupling them to pathways such as PI3K/AKT and MAPK/ERK that control cell growth and differentiation.
• Through localized modulation of Rho family GTPases via GEFs and GAPs, FAK orchestrates actin cytoskeleton reorganization, cell spreading, and formation of cell protrusions.
• FAK directly phosphorylates NEDD9 (HEF1) upon integrin engagement, contributing to downstream signaling complex assembly. Related references: PMID:9360983
• The non-catalytic isoform 6 (FRNK) acts as a dominant-negative inhibitor of FAK signaling, attenuating nuclear LPXN accumulation and serum response factor-dependent transcription.
• Subcellularly, FAK dynamically localizes to focal adhesions, the cell cortex, perinuclear cytoplasm, centrosomes, and the nucleus, reflecting its multifaceted roles in signal transduction and structural organization.
• Post‑translational modifications, including phosphorylation on multiple tyrosines, serines, and threonines, as well as acetylation and ubiquitination, tightly regulate FAK activity, stability, and protein interactions.
• Ubiquitously expressed with enrichment in epithelial cells, lymphocytes, and lung fibroblasts, FAK is indispensable for embryonic development, angiogenesis, and nervous system maturation.

Experimental Guidance and Technical Tips

• The immunogen targets the phosphorylated Y397 residue; to confirm phospho-specificity in WB, consider treating samples with lambda phosphatase or incubating the antibody with a non‑phosphorylated blocking peptide.
• For ELISA applications, using the phosphorylated peptide as a positive control and its non‑phosphorylated counterpart as a negative control can help validate signal specificity.
• FAK migrates at approximately 119 kDa on SDS-PAGE; ensure proper gel percentage and extended transfer times for high-molecular-weight blots.
• This antibody was generated against the human FAK sequence; cross-reactivity with other species has not been extensively tested. Validate in your specific model system if used outside human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated sourcing partner for IVD manufacturers, offering the above anti‑FAK‑Y397 monoclonal antibody alongside validated antibody pairs, matched recombinant proteins, and bulk ancillary reagents. Whether you are expanding an immunoassay panel for cell adhesion research or scaling up production, our team streamlines procurement from early‑stage development to commercial supply, including rare‑target sourcing. With deep expertise in signaling targets, CamelBio bridges your diagnostic needs from concept to clinic.