Phospho-TP73-Y99 Rabbit Polyclonal Antibody for WB, ELISA - O15350

Core Product Specifications and Parameters

Biological Background: TP73 Function and Localization

• TP73 encodes tumor protein p73, a member of the p53 family of transcription factors. It shares structural and functional homology with p53, including DNA-binding and transactivation domains, and exists in multiple isoforms generated by alternative promoter usage and splicing (UniProt: O15350).
• The transactivation-competent isoforms (TAp73) function as pro-apoptotic factors and tumor suppressors, participating in the DNA damage response. In contrast, N-terminally truncated isoforms (ΔNp73) lacking the transactivation domain inhibit p53 and TAp73 functions, acting as anti-apoptotic regulators.
• TAp73 is an essential positive regulator of lung ciliated cell differentiation by activating FOXJ1 expression. This role is critical for respiratory epithelial function and motile ciliogenesis. Related references: PMID:34077761
• Subcellular localization of p73 is predominantly nuclear, consistent with its transcription factor activity, though it can also be detected in the cytoplasm. Shuttling between compartments may regulate its accessibility and function.
• p73 is broadly expressed in human tissues, including brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus, pancreas, and lung, with elevated levels in fetal tissues. At the protein level, it is detected in striatal neurons of Huntington disease patients and in the respiratory epithelium.
• Post-translational modifications, particularly phosphorylation (e.g., at tyrosine 99), modulate p73 stability, transcriptional activity, and interactions with upstream kinases and binding partners, linking it to cell cycle control and apoptosis signaling.
• Keywords associated with TP73 highlight its involvement in apoptosis, cell cycle, transcription regulation, tumor suppression, host-virus interactions, and its link to diseases such as primary ciliary dyskinesia and lissencephaly.

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide corresponding to residues surrounding Y99 of human TP73. For Western blot, consider using a phospho-blocking peptide competition assay to confirm phosphorylation-dependent detection.
• Given the role of TP73 in DNA damage response, treating cells with genotoxic agents (e.g., etoposide, cisplatin) may enhance phosphorylation at Y99 and improve signal in WB.
• The predicted molecular weight of full-length p73 is approximately 70 kDa, but multiple isoforms exist. Validate band identity by comparing treated vs untreated samples and using appropriate molecular weight markers.
• For ELISA applications, pairing this antibody with a capture antibody recognizing a separate epitope may increase sensitivity. Include phosphorylated and non-phosphorylated peptide controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials for TP73-related research, including validated antibodies for apoptosis and DNA damage signaling. From monoclonal and polyclonal antibody pairs to bulk ancillary reagents, we support your assay development needs with one-stop sourcing and expert technical consultation. Contact us to discuss custom requirements for rare or phospho-specific targets like phospho-TP73-Y99.