Phospho-LCK-Y394 Rabbit pAb - P06239

Core Product Specifications and Parameters

Biological Background: LCK Function and Localization

• LCK is a non-receptor tyrosine-protein kinase essential for T-cell selection, maturation in the thymus, and mature T-cell function.
  Related references: PMID:2470098
• Upon T-cell receptor (TCR) engagement with peptide-MHC, LCK is recruited to the TCR-CD3 complex and phosphorylates tyrosine residues within ITAMs of CD3 and TCR chains, triggering ZAP70 recruitment and downstream lymphokine production.
  Related references: PMID:2470098 PMID:40592325
• LCK also participates in signaling via CD2 and IL2 receptors: CD2 association leads to LCK hyperphosphorylation, and IL2 binding enhances LCK activity to promote T-cell proliferation.
• It phosphorylates multiple substrates including RUNX3, PTK2B/PYK2, MAPT, RHOH, and TYROBP, and interacts with FYB2.
  Related references: PMID:27335501
• Localizes to the cell membrane, cytoplasm, and cytosol.
• Expressed specifically in lymphoid cells.
• Annotated with keywords such as alternative splicing, ATP-binding, kinase, phosphoprotein, proto-oncogene, SH2 and SH3 domains, and lipidation (myristate and palmitate), reflecting its multifaceted regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Tyr394, so the antibody is expected to specifically recognize LCK phosphorylated at this activation loop site.
• For WB, use phosphatase inhibitors during sample preparation to preserve phosphorylation. A band at ~58 kDa is anticipated in lymphoid cell lysates from human and mouse.
• Validate antibody performance in appropriate T-cell models (e.g., Jurkat cells) stimulated through CD3/CD28 to induce robust Tyr394 phosphorylation.
• IF/ICC may require optimized fixation/permeabilization; consider testing in activated T-cells or thymus tissue sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and research labs with ready access to validated antibodies and ancillary reagents. This anti-phospho-LCK-Y394 antibody supports T-cell signaling and immunology research, and we can complement your projects with bulk reagent supply, custom antibody development, and rare-target sourcing to accelerate assay development from concept to clinic.