Phospho-Myosin Light Chain 2-S19 Rabbit mAb for WB, ELISA - P24844

Core Product Specifications and Parameters

Biological Background: Myosin Light Chain 2 Function and Localization

• Functions as a regulatory myosin subunit, modulating smooth muscle and nonmuscle cell contractility upon phosphorylation. It is involved in essential cellular functions such as cytokinesis, receptor capping, and cell locomotion. Related references: PMID:11942626 | PMID:2526655
• In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly, contributing to myotube formation (By similarity).
• Primarily localizes to the cytoplasm, associating with the cytoskeleton and cell cortex.
• Predominantly expressed in smooth muscle tissues and in some nonmuscle cells.
• Undergoes regulatory phosphorylation at Ser19, along with other post-translational modifications including acetylation; also contains calcium-binding EF-hand motifs.
• Listed under keywords: Acetylation, Alternative splicing, Calcium, Cytoplasm, Cytoskeleton, Metal-binding, Motor protein, Muscle protein, Myosin, Phosphoprotein, Proteomics identification, Reference proteome, Repeat.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to Ser19 of human Myosin Light Chain 2. This design enables specific detection of the phosphorylated form; confirm specificity by testing with phosphatase-treated samples.
• For Western blotting, validate the antibody with appropriate positive controls (e.g., stimulated smooth muscle cells or tissues) to ensure detection of the ~20 kDa band.
• Cross-reactivity with mouse and rat is expected based on sequence homology; verify performance in your experimental system.
• For ELISA applications, a phosphopeptide blocking experiment may be considered to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers, labs, and research institutes with a one-stop supply chain from concept to clinic. For researchers exploring myosin light chain 2 phosphorylation in smooth muscle contractility, cell migration, or cytoskeletal dynamics, we provide validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents—streamlining your development of immunoassays and diagnostic kits. Additionally, we offer customized sourcing services for rare-target raw materials, ensuring continuity from discovery to commercialization.