Phospho-LATS1-T1079+LATS2-T1041 Rabbit Monoclonal Antibody - O95835 / Q9NRM7

Core Product Specifications and Parameters

Biological Background: LATS1/LATS2 Function and Localization

• Serine/threonine-protein kinase LATS1 (Large tumor suppressor homolog 1) is a core component of the Hippo signaling pathway, which regulates organ size and suppresses tumors by restricting proliferation and promoting apoptosis.
• In the Hippo kinase cascade, MST1/STK4 and MST2/STK3, complexed with SAV1, phosphorylate LATS1/2 in complex with MOB1; activated LATS1/2 then phosphorylates and inactivates the oncoproteins YAP1 and WWTR1/TAZ, preventing their nuclear translocation and transcription of pro-proliferative genes. Related references: PMID:10518011, PMID:10831611, PMID:18158288, PMID:26437443, PMID:28068668
• LATS1 also functions as a tumor suppressor in maintaining ploidy by regulating mitotic progression and the G1 tetraploidy checkpoint, and it negatively regulates G2/M transition by down-regulating CDK1 activity. Related references: PMID:15122335, PMID:19927127, PMID:9988268
• Beyond kinase signaling, LATS1 modulates the cytoskeleton during cytokinesis by inhibiting LIMK1, affecting actin polymerization. Related references: PMID:15220930
• LATS1 is localized to centrosomes, spindle poles, midbody, and the cytoplasm, consistent with its roles in mitosis and cytoskeletal regulation.
• The protein is broadly expressed in adult tissues except lung and kidney, and its activity is regulated by phosphorylation—the T1079 residue (in LATS1; T1041 in LATS2) is a key activation site within the kinase activation loop.
• Keywords include tumor suppressor, kinase, phosphorylation, cell cycle, and mitosis. Note: LATS2 is a paralog with similar domain organization and overlapping functions; the antibody targets the corresponding phospho-site in both kinases.
• Additionally, LATS1 has been shown to activate the NLRP3 inflammasome by phosphorylating NLRP3 at Ser-265, linking Hippo signaling to innate immunity. Related references: PMID:39173637

Experimental Guidance and Technical Tips

• For western blot (WB), expect bands at ~126 kDa for LATS1 and ~76 kDa for LATS2 in human, mouse, or rat samples. Validate phospho-specificity by treating with phosphatase or using non‑phospho controls.
• For IF/ICC and IHC-P, the antibody may detect centrosomal, spindle, or midbody localization in mitotic cells; consider co‑staining with tubulin or γ‑tubulin markers.
• ELISA can be used for quantitative detection of phosphorylated LATS1/2 in cell or tissue lysates; follow standard phospho‑ELISA protocols.
• Because the immunogen is a peptide common to both LATS1 and LATS2, the antibody recognizes both kinases; confirm target identity by siRNA knockdown or isoform‑specific antibodies if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This anti‑phospho‑LATS1/2 antibody supports research into Hippo signaling, tumor suppression, and cell cycle regulation. For bulk orders, custom formulations, or sourcing of rare‑target reagents, CamelBio offers expert technical support and global supply chain solutions.