Phospho-FAK-Y397 Rabbit Polyclonal Antibody for WB, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• Focal adhesion kinase 1 (FAK, also known as PTK2) is a non-receptor protein-tyrosine kinase that plays a central role in integrin-mediated signal transduction, regulating cell migration, adhesion, spreading, and cytoskeletal reorganization.
• Encoded by the PTK2 gene, FAK is essential for early embryonic development, angiogenesis, cardiomyocyte function, and nervous system development (axon growth, neuronal migration, synapse formation).
• FAK undergoes autophosphorylation at Tyr397 upon activation, creating a high-affinity binding site for SRC family kinases; this event is critical for assembling signaling complexes that propagate downstream pathways such as PI3K/AKT and MAPK/ERK.
• Subcellularly, FAK localizes predominantly to focal adhesions, but also to the cell membrane, perinuclear cytoplasm, cell cortex, cytoskeleton, centrosome, cilium basal body, and nucleus, reflecting its diverse signaling roles.
• Isoform 6 (FRNK) lacks the kinase domain and functions as a dominant-negative inhibitor of FAK signaling, attenuating nuclear accumulation of LPXN and limiting SRF-dependent gene transcription.
• FAK is widely expressed; detected ubiquitously and especially in epithelial cells, B and T lymphocytes, and lung fibroblasts. Related references: PMID:31630787
• Key post-translational modifications include tyrosine phosphorylation (e.g., Y397), acetylation, and ubiquitination, which regulate its kinase activity, protein interactions, and stability.
• This antibody specifically recognizes FAK phosphorylated at Tyr397, enabling researchers to monitor FAK activation status in signaling studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Tyr397; the antibody is expected to specifically recognize the phosphorylated form. Validate specificity by treating lysates with phosphatase or using non-phosphorylated peptide blocking.
• For Western blot, the predicted molecular weight is ~119 kDa. Use appropriate positive controls (e.g., pervanadate-treated cells to induce phosphorylation).
• For ELISA, consider coating plates with the phospho-peptide and using a total FAK antibody as a loading control.
• Cross-reactivity with mouse and rat is reported, but perform titration experiments in the relevant model system.
• Shipment on ice bags; store at -20°C upon receipt for long-term stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supplying research-grade antibodies like this phospho-FAK (Y397) antibody for cell adhesion and developmental biology studies. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and manufacturing workflows.