Anti-Phospho-ZAP70-Y319 Rabbit Polyclonal Antibody for WB, IP, ELISA - P43403

Core Product Specifications and Parameters

Biological Background: ZAP70 Function and Localization

• ZAP-70 (70 kDa zeta-chain associated protein) is a cytoplasmic tyrosine kinase encoded by the ZAP70 (SRK) gene and belongs to the Syk family of non-receptor tyrosine kinases.
• Essential for adaptive immunity, ZAP-70 is recruited to the phosphorylated T-cell receptor (TCR) complex at the plasma membrane via its SH2 domains, initiating downstream signaling. Related references: PMID:7509083
• Upon TCR engagement, ZAP-70 phosphorylates key adaptors LAT and LCP2, triggering cascades that lead to T-cell activation, proliferation, and cytokine production.
• Regulates thymocyte development, promoting survival and cell-cycle progression of double-negative thymocytes prior to positive selection.
• Also involved in primary B-cell development and activation through B-cell receptor (BCR) signaling.
• Localized in the cytoplasm and at the cell membrane, ZAP-70 undergoes phosphorylation at Y319 (among other residues) to relieve autoinhibition and enable full kinase activity.
• Phosphorylation, acetylation, and ubiquitination are critical post-translational modifications regulating ZAP-70 stability and function.
• Expressed predominantly in T-cells, natural killer cells, early thymocytes, and pro/pre-B cells.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide corresponding to Y319 of human ZAP70; confirm specificity by peptide competition assay if required.
• For Western blotting, use validated phospho-specific protocols and include phosphatase inhibitors in lysis buffers to preserve phosphorylation states.
• In immunoprecipitation experiments, mild lysis conditions are recommended to maintain protein-protein interactions and epitope accessibility.
• Validate performance in appropriate human T-cell lines (such as Jurkat) activated via anti-CD3/CD28 to induce ZAP70 phosphorylation.
• For ELISA applications, titrate antibody concentration against a phospho-peptide standard to ensure a linear detection range.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop IVD raw material partner, CamelBio supplies high-quality phospho-specific antibodies like this ZAP70-Y319 antibody to support T-cell signaling and immune research projects. We offer validated antibody pairs, bulk ancillary reagents, and sourcing solutions for rare-target raw materials, helping diagnostic manufacturers accelerate from concept to clinic. Contact us to explore custom procurement and technical support for your IVD development needs.