Anti-Fyn Rabbit Monoclonal Antibody for WB, ELISA - P06241

Core Product Specifications and Parameters

Biological Background: Fyn Function and Localization

• Fyn is a non-receptor tyrosine-protein kinase belonging to the Src family, also known as proto-oncogene Syn, c-Fyn, SLK, and p59-Fyn. Two main isoforms exist: isoform 1 is highly expressed in the brain, while isoform 2 is predominantly found in hemopoietic lineages, especially T-lymphocytes.
• It plays a central role in regulating cell growth, survival, adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response, and axon guidance. Related references: PMID:11536198, PMID:15489916, PMID:15557120
• Fyn is activated by dephosphorylation of its C-terminal inhibitory tyrosine and subsequent PKA-mediated phosphorylation, leading to association with PTK2/FAK1 and targeting to focal adhesions. Related references: PMID:15489916
• In cell adhesion and motility, Fyn phosphorylates CTNNB1 (β-catenin) and CTNND1 (δ-catenin). Related references: PMID:17194753
• It regulates cytoskeletal remodeling by phosphorylating actin regulator WAS and microtubule-associated proteins MAP2 and MAPT, and promotes cell survival via phosphorylation of AGAP2/PIKE-A. Related references: PMID:14707117, PMID:15536091, PMID:16841086
• In the kidney, Fyn maintains glomerular filtration barrier integrity by phosphorylating slit diaphragm components NPHS1, KIRREL1, and TRPC6. Related references: PMID:14761972, PMID:18258597, PMID:19179337
• Neural functions include phosphorylation of DPYSL2, ARHGAP32, and SNCA, contributing to axon guidance and synaptic regulation. Related references: PMID:11162638, PMID:12788081, PMID:19652227
• In immune cells, Fyn mediates T-cell receptor signaling by phosphorylating PTK2B/PYK2, and controls negative feedback via PAG1 and PDCD1 phosphorylation. It also promotes CD28-induced VAV1 phosphorylation and CD244-mediated NK cell activation. Related references: PMID:22080863, PMID:20028775, PMID:18056706, PMID:32184441, PMID:11005864, PMID:15713798
• Fyn localizes to the cytoplasm, nucleus, cell membrane, and perikaryon.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Fyn, which may allow detection of both major isoforms (isoform 1 and isoform 2). Verify isoform specificity in your experimental system.
• For western blotting (WB), a band around 61 kDa is expected. However, post-translational modifications and isoform expression patterns may affect migration; validate in relevant sample lysates.
• In ELISA applications, consider using this antibody as a capture or detection reagent after confirming performance with your target antigen and buffer conditions.
• Cross-reactivity with mouse and rat has been reported. If working with these species, confirm epitope conservation and run appropriate controls to avoid non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with integrated access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-Fyn rabbit mAb. Covering every stage from concept to clinic, we support your tyrosine kinase signaling, immune response, or neuroscience research with bulk ancillary reagents and rare target sourcing.