Phospho-Ezrin-Y353 Rabbit pAb for WB and ELISA - P15311

Core Product Specifications and Parameters

Biological Background: Ezrin Function and Localization

• Ezrin, also known as Cytovillin, Villin-2, and p81, is encoded by the EZR gene (GeneID: 7430).
• Functions as a linker between the plasma membrane and the actin cytoskeleton, essential for maintaining cell shape and polarity.
• Involved in the formation of microvilli and membrane ruffles at the apical surface of epithelial cells.
• Along with PLEKHG6, required for normal macropinocytosis.
• Localizes to apical cell membrane, microvillus membrane, ruffle membrane, and the cortical cytoskeleton.
• Expressed predominantly in cerebral cortex, hippocampus, and intestinal epithelial microvilli, with preferential expression in astrocytes.
• Subject to post-translational modifications including phosphorylation at Y353, which may regulate its activation and membrane-cytoskeletal linkage.

Experimental Guidance and Technical Tips

• The immunogen is designed around the Y353 phosphorylation site; consider using phosphatase inhibitors during lysis to preserve the phospho-epitope.
• For Western blot, validate the antibody in human cell lines or tissues known to express Ezrin; a band around 69 kDa is expected.
• In ELISA applications, optimize blocking and antibody dilution conditions to minimize non-specific binding due to the phospho-specific nature of the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering this anti-Phospho-Ezrin (Y353) antibody to support cytoskeleton and cell biology research. Our platform provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your workflow from concept to clinic.