Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Rabbit pAb for WB, ELISA - P15311 / P35241 / P26038

Core Product Specifications and Parameters

Biological Background: ERM Family Protein (Ezrin/Radixin/Moesin) Function and Localization

• Ezrin (Cytovillin, Villin-2, p81) is a member of the ERM (ezrin-radixin-moesin) protein family, which links the actin cytoskeleton to the plasma membrane.
• It is involved in the formation of microvilli and membrane ruffles at the apical pole of epithelial cells, and, together with PLEKHG6, is required for normal macropinocytosis.
• Subcellular localizations include the apical cell membrane, microvillus membrane, ruffle membrane, cytoplasm, cell cortex, and cytoskeleton.
• Ezrin is expressed in the cerebral cortex, basal ganglia, hippocampus, hypophysis, optic nerve, and intestinal epithelial microvilli; preferentially in astrocytes of the brain.
• Post‑translational modifications include phosphorylation, acetylation, and S‑nitrosylation (UniProt keywords: Phosphoprotein, Acetylation, S‑nitrosylation).

Experimental Guidance and Technical Tips

• For Western blotting, use fresh samples treated with phosphatase inhibitors to preserve the phosphorylation signal; the antibody may detect bands around 68‑69 kDa for ERM proteins, but the observed molecular weight can range from 23‑71 kDa depending on isoforms or cleavage products.
• This polyclonal antibody is raised against a synthetic phosphopeptide; validate specificity by treating samples with phosphatase to confirm loss of signal.
• For ELISA applications, consider using the antibody as a capture or detection reagent against phosphorylated ERM family peptides or recombinant proteins; optimize coating and detection conditions accordingly.
• Due to high homology among ERM family members, this antibody recognizes the phosphorylated forms of Ezrin, Radixin, and Moesin; verify reactivity in your specific experimental system.

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