Phospho-EZH2/KMT6 (T311) Rabbit Polyclonal Antibody for WB, ELISA - Q15910

Core Product Specifications and Parameters

Biological Background: EZH2 Function and Localization

• EZH2 is a histone-lysine N-methyltransferase, also known as enhancer of zeste homolog 2, ENX-1, or KMT6, and functions as the catalytic subunit of the PRC2 complex.
• The PRC2/EED-EZH2 complex methylates histone H3 at Lys-9 and Lys-27, leading to transcriptional repression of target genes. Related references: PMID:14532106, PMID:15225548, PMID:15385962
• EZH2 mono-, di-, and trimethylates H3K27, with a preference for unmethylated substrate (H3K27me0) and progressively lower activity on H3K27me1 and H3K27me2. Related references: PMID:15231737, PMID:17210787, PMID:18285464, PMID:22323599, PMID:30923826
• In embryonic stem cells, EZH2 is more abundant than EZH1 and is essential for establishing H3K27me3 marks required for stem cell identity and proper differentiation. Related references: PMID:19026781
• The PRC2 complex may recruit DNA methyltransferases, linking histone methylation to DNA methylation and reinforcing gene silencing. Related references: PMID:16357870, PMID:17200670
• Known target genes repressed by EZH2/PRC2 include HOXC8, HOXA9, MYT1, CDKN2A, and retinoic acid-responsive genes. Related references: PMID:16179254, PMID:18086877, PMID:20935635
• EZH2 also methylates non-histone proteins such as the transcription factor GATA4 and nuclear receptor RORA, expanding its regulatory scope. Related references: PMID:23063525
• It regulates circadian rhythms by methylating histone H3 at promoters of clock genes, facilitating CRY1/2-mediated repression of PER1/2 transcription.
• EZH2 is predominantly localized in the nucleus and is widely expressed in many tissues; overexpression is frequently observed in breast, colon, larynx, lymphoma, and testicular cancers. Related references: PMID:14532106
• EZH2 is a phosphoprotein, and phosphorylation at residue Thr311 is one of the post-translational modifications that may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide carrying phosphorylation at Thr311; therefore, the antibody is expected to specifically recognize the phosphorylated form of EZH2 at this site. Confirm specificity by comparing signals in phosphatase-treated versus untreated samples.
• For western blot (WB), EZH2 migrates at ~85kDa. Use appropriate positive controls such as HEK293T or HeLa cells, and validate detection of the phospho-T311 form under conditions that induce EZH2 phosphorylation.
• ELISA can be employed for quantitative analysis of phospho-EZH2 (T311) levels; consider using a capture antibody in combination with this pAb for sandwich ELISA.
• Cross-reactivity with rat has been observed; thus, this antibody may be suitable for rodent model studies. Validate in the relevant tissue or cell line system to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, with this phospho-EZH2 (T311) rabbit polyclonal antibody serving as a specialized tool for epigenetic and chromatin research. We provide one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your diagnostic and research workflow.