Anti-Phospho-ErbB3/HER3-Y1289 Rabbit Monoclonal Antibody for WB, ELISA - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3 Function and Localization

• Receptor tyrosine-protein kinase erbB-3 (ErbB3/HER3) functions as a cell surface receptor for neuregulins, particularly NRG1, leading to receptor phosphorylation and PI3K recruitment. Related references: PMID:20682778
• Alternative activation may occur through CSPG5 binding. Related references: PMID:15358134
• ErbB3 is involved in the regulation of myeloid cell differentiation. Related references: PMID:27416908
• Localizes primarily at the cell membrane; a secreted form is also produced, likely from alternative splicing.
• Expressed in epithelial tissues and brain, consistent with its roles in development and cancer.
• Undergoes ligand-dependent tyrosine phosphorylation and is a glycoprotein containing disulfide bonds, reflecting its extracellular domain structure.
• Alternative splicing generates multiple isoforms, including secreted variants p45-sErbB3 and p85-sErbB3, which may modulate receptor activity.
• UniProt classifies ERBB3 with the 'Disease variant' keyword, indicating its potential involvement in various pathologies.

Experimental Guidance and Technical Tips

• This phospho-specific monoclonal antibody detects ErbB3 phosphorylated at Y1289. For optimal results in WB, include phosphatase inhibitors in lysis buffer and consider inducing ErbB3 phosphorylation with neuregulin.
• Validate antibody specificity by parallel treatment with lambda phosphatase or by using non-phosphorylated peptide competition. Unstimulated versus ligand-treated cell lysates can confirm phosphorylation-dependent detection.
• For ELISA, appropriate antigen coating and detection conditions should be optimized. The antibody may be suitable for capture or detection in sandwich immunoassays; pair with a total ErbB3 antibody for phospho/total ratio analysis.
• Cross-reactivity with other ErbB family members or non-specific binding should be assessed using relevant knockout or knockdown controls in the intended sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including phospho-specific antibodies like this anti-ErbB3 (Y1289) monoclonal antibody. Our offerings extend to validated antibody pairs, bulk ancillary reagents, and sourcing for hard-to-find targets, streamlining your assay development from concept to clinic. With expertise in receptor tyrosine kinase signaling, we provide the materials you need to advance precision diagnostics and targeted therapy research.