Anti-Phospho-ErbB3/HER3-Y1328 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21860

Core Product Specifications and Parameters

Biological Background: Receptor tyrosine-protein kinase erbB-3 (ErbB3/HER3) Function and Localization

• Receptor tyrosine-protein kinase erbB-3 (ErbB3/HER3), also known as proto-oncogene-like protein c-ErbB-3, is a member of the epidermal growth factor receptor (EGFR/ErbB) family and is encoded by the ERBB3 gene.
• It functions as a cell surface receptor for neuregulins, primarily NRG1; ligand binding induces tyrosine phosphorylation and promotes association with the p85 subunit of PI3K, activating downstream signaling pathways (PubMed:20682778). Related references: PMID:20682778
• ErbB3 may also be activated by CSPG5, expanding its role in neural development (PubMed:15358134). Related references: PMID:15358134
• Subcellular localization includes the cell membrane and secreted forms; alternative splicing generates multiple isoforms, including secreted variants.
• Tissue specificity is primarily in epithelial tissues and brain, consistent with its roles in development and cancer.
• Key post-translational modifications include phosphorylation at multiple tyrosine residues (e.g., Y1328), glycosylation, and disulfide bond formation; it is a phosphoprotein and glycoprotein.
• It is involved in the regulation of myeloid cell differentiation, highlighting a role in immune cell development (PubMed:27416908). Related references: PMID:27416908
• ErbB3 is frequently associated with disease variants, including cancers, and is a target for therapeutic interventions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1328, ensuring site-specificity for the phosphorylated form of ErbB3. Use phosphatase inhibitors during sample preparation for Western blotting.
• For Western blot (WB), a protein weight of ~148 kDa is expected; consider using appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols to preserve phospho-epitopes.
• Validate the antibody in cell lines or tissues known to express ErbB3 (human, mouse, rat) and treat with neuregulin to induce phosphorylation for positive controls.
• ELISA applications may require optimizing coating antigen and detection conditions; consider using phosphorylated peptide versus non-phosphorylated peptide to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This phospho-specific ErbB3/HER3 antibody is part of our extensive portfolio for cancer and signaling research, complemented by validated antibody pairs, optimised monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our integrated supply chain also facilitates customised sourcing of rare-target raw materials, ensuring you can advance your projects with confidence and efficiency.