Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb for WB, ELISA - P15311 / P35241 / P26038

Core Product Specifications and Parameters

Biological Background: Ezrin Function and Localization

• Ezrin, also known as cytovillin, villin-2, or p81, is a member of the ERM (Ezrin/Radixin/Moesin) protein family that links the actin cytoskeleton to the plasma membrane.
• It plays a critical role in epithelial cell polarity, contributing to the formation of microvilli and membrane ruffles at the apical surface, and is required for normal macropinocytosis together with PLEKHG6.
• The ERM family includes Ezrin, Radixin, and Moesin, which share conserved phosphorylation sites (e.g., T567/T564/T558) that regulate their active conformation.
• Ezrin localizes to the apical cell membrane, microvillus and ruffle membranes, as well as the cell cortex and underlying cytoskeleton.
• Expression is prominent in the cerebral cortex, hippocampus, and intestinal epithelial microvilli, with preferential astrocyte localization in the brain and absence from most neurons.
• Post‑translational modifications include phosphorylation (notably at T567 which activates the protein), acetylation, and S‑nitrosylation, regulating its conformation and membrane-cytoskeleton linking activity.
• The protein contains a coiled-coil domain and has a molecular weight of approximately 69 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, use phosphatase inhibitors in lysis buffers to preserve phosphorylation signals. Expect bands between 23-71 kDa corresponding to different ERM family members.
• Validate antibody specificity by using non-phosphorylated peptide blocking or phosphatase treatment controls in your sample system.
• For ELISA, the phosphorylated immunogen peptide may serve as a positive control; consider testing on stimulated versus unstimulated cell lysates.
• Species cross-reactivity is confirmed for human and rat; additional species require experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with comprehensive access to IVD raw materials, offering this anti-phospho-ERM antibody alongside validated antibody pairs, bulk ancillary reagents, and rare-target sourcing capabilities. Whether you need optimized phosphorylated antibodies for biomarker studies or large-scale production reagents, our supply network is designed to bridge the gap from concept to clinic. We welcome inquiries about custom solutions tailored to your cytoskeleton and cell signaling research projects.