Phospho-Ezrin-T567 Rabbit pAb for WB - P15311

Core Product Specifications and Parameters

Biological Background: Ezrin Function and Localization

• Ezrin (also known as Cytovillin, Villin-2, p81) is a ~69 kDa cytoskeletal linker protein encoded by the EZR (VIL2) gene.
• It functions as a critical connector between the plasma membrane and the actin cytoskeleton, playing an essential role in the formation of epithelial microvilli and membrane ruffles at the apical cell surface.
• Together with the guanine nucleotide exchange factor PLEKHG6, ezrin is required for normal macropinocytosis, a process of non-selective fluid-phase endocytosis.
• Subcellularly, ezrin localizes to the apical cell membrane, microvillus and ruffle membranes, as well as the cell cortex and cytoskeleton, reflecting its dynamic role in membrane-cytoskeleton remodeling.
• Tissue expression is prominent in the microvilli of intestinal epithelial cells and preferentially in astrocytes of the hippocampus, frontal cortex, and other brain regions; it is weakly expressed or absent in neurons of most tissues studied.
• Post-translational modifications include phosphorylation (a hallmark of its activation), acetylation, and S-nitrosylation, all contributing to its regulatory versatility in cellular processes such as cell shape maintenance, adhesion, and signaling.

Experimental Guidance and Technical Tips

• Since this antibody is phospho-specific (T567), ensure thorough inhibition of phosphatases during cell/tissue lysis (e.g., using phosphatase inhibitor cocktails) to preserve the phosphorylation signal.
• For Western blot, a band at approximately 69 kDa is expected; consider using a positive control such as pervanadate-treated cells to verify phospho-specificity.
• To confirm the phospho-dependence of the signal, treat samples with lambda protein phosphatase or perform peptide competition assays with the non-phosphorylated immunogen peptide.
• While optimized for human samples, the reported cross-reactivity with mouse and rat suggests high sequence conservation; validate the antibody in your specific model system using relevant controls.
• This antibody is recommended for Western blot (WB) only; its performance in other applications (e.g., IHC, IF, IP) has not been assessed and would require further validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering a broad catalog of high-quality IVD raw materials and custom reagent solutions. For cytoskeletal research and cell biology applications, we provide validated primary antibodies like this phospho-ezrin (T567) antibody, alongside optimized ancillary reagents and bulk ordering options. Whether you need monoclonal antibody pairs, recombinant proteins, or rare-target raw materials, CamelBio streamlines your supply chain from concept to clinic.