Anti-Phospho-EGFR-Y1172 Rabbit pAb for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that, upon ligand binding, activates key signaling pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STAT modules, thereby regulating cell proliferation, differentiation, and survival. Related references: PMID:10805725, PMID:27153536, PMID:2790960
• Recognized ligands include EGF, TGFA, AREG, EPGN, BTC, EREG, and HBEGF, which trigger receptor homo- or heterodimerization and autophosphorylation on cytoplasmic tyrosine residues. Related references: PMID:12297049, PMID:15611079, PMID:17909029
• In addition to canonical pathways, EGFR directly phosphorylates RGS16 (activating its GTPase activity) and MUC1 (enhancing its interaction with SRC and beta-catenin), linking it to G-protein coupled receptor signaling and cell adhesion. Related references: PMID:11602604, PMID:11483589
• EGFR also activates NF-kappa-B signaling and interacts with CCDC88A/GIV at the cell membrane to promote sustained cell migration. Related references: PMID:11116146, PMID:20462955
• Isoform 2, generated by alternative splicing, lacks kinase activity and may act as an antagonist of EGF action; it is notably expressed in ovarian cancers.
• Subcellularly, EGFR localizes to the cell membrane, endoplasmic reticulum, Golgi apparatus, endosomes, nucleus, and can be secreted, reflecting its dynamic trafficking and signaling roles.
• Ubiquitously expressed in human tissues, EGFR is classified as a phosphoprotein, tyrosine-protein kinase, and proto-oncogene, and serves as a host cell receptor for hepatitis C virus entry.

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide surrounding Y1172; ensure that lysis buffers contain phosphatase inhibitors to preserve phosphorylation.
• For Western blot, expect a band at approximately 134 kDa under standard reducing conditions; use appropriate molecular weight markers.
• When performing ELISA, the immunogenic phospho-peptide can serve as a positive control to validate antibody specificity.
• Validate in the relevant cell line or tissue system with known EGFR expression and phosphorylation status, using untreated vs. EGF-stimulated samples where possible.

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